Micropropagation and genetic stability of a Dendrobium hybrid (Orchidaceae)
Tipo
Artigo
Data de publicação
2006
Periódico
In Vitro Cellular and Developmental Biology - Plant
Citações (Scopus)
35
Autores
Ferreira W.D.M.
Kerbauy G.B.
Costa A.P.P.
Kerbauy G.B.
Costa A.P.P.
Orientador
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
Dendrobium hybrids have great economic importance in a number of countries. Asymbiotic seed germination and the conventional vegetative method have been commonly used by growers to propagate these plants. To overcome somaclonal variation, which is commonly exhibited by Dendrobium (Nobile group) when micropropagated from protocorm-like bodies, a protocol for propagating Dendrobium Second Love in vitro using axillary buds in the presence of thidiazuron was developed. Random amplified polymorphic DNA analysis was also carried out to check for possible genetic alterations in plants originating from six consecutive subcultures. The results revealed that the established protocol was efficient for the in vitro cloning of this orchid hybrid and the plants obtained from the six subcultures did not exhibit any type of polymorphism. © 2006 Society for In Vitro Biology.