Micropropagation and genetic stability of a Dendrobium hybrid (Orchidaceae)

dc.contributor.authorFerreira W.D.M.
dc.contributor.authorKerbauy G.B.
dc.contributor.authorCosta A.P.P.
dc.date.accessioned2024-03-13T01:41:41Z
dc.date.available2024-03-13T01:41:41Z
dc.date.issued2006
dc.description.abstractDendrobium hybrids have great economic importance in a number of countries. Asymbiotic seed germination and the conventional vegetative method have been commonly used by growers to propagate these plants. To overcome somaclonal variation, which is commonly exhibited by Dendrobium (Nobile group) when micropropagated from protocorm-like bodies, a protocol for propagating Dendrobium Second Love in vitro using axillary buds in the presence of thidiazuron was developed. Random amplified polymorphic DNA analysis was also carried out to check for possible genetic alterations in plants originating from six consecutive subcultures. The results revealed that the established protocol was efficient for the in vitro cloning of this orchid hybrid and the plants obtained from the six subcultures did not exhibit any type of polymorphism. © 2006 Society for In Vitro Biology.
dc.description.firstpage568
dc.description.issuenumber6
dc.description.lastpage571
dc.description.volume42
dc.identifier.doi10.1079/IVP2006820
dc.identifier.issn1054-5476
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37747
dc.relation.ispartofIn Vitro Cellular and Developmental Biology - Plant
dc.rightsAcesso Restrito
dc.subject.otherlanguageIn vitro propagation
dc.subject.otherlanguageOrchid
dc.subject.otherlanguageRandom amplified polymorphic DNA (RAPD)
dc.subject.otherlanguageThidiazuron
dc.titleMicropropagation and genetic stability of a Dendrobium hybrid (Orchidaceae)
dc.typeArtigo
local.scopus.citations35
local.scopus.eid2-s2.0-34250881000
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34250881000&origin=inward
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