Expression of recombinant Araraquara Hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in Escherichia coli

MacHado A.M.; MacHado A.R.S.R.; Moreli M.L.; Ribeiro B.M.; Figueiredo L.T.M.; Wolff J.L.C.

Expression of recombinant Araraquara Hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in Escherichia coli

Tipo

Artigo

Data de publicação

2011

Periódico

Virology Journal

Citações (Scopus)

3

Autores

MacHado A.M.
MacHado A.R.S.R.
Moreli M.L.
Ribeiro B.M.
Figueiredo L.T.M.
Wolff J.L.C.

Resumo

Background: Antigens for Hantavirus serological tests have been produced using DNA recombinant technology for more than twenty years. Several different strategies have been used for that purpose. All of them avoid the risks and difficulties involved in multiplying Hantavirus in the laboratory. In Brazil, the Araraquara virus is one of the main causes of Hantavirus Cardio-Pulmonary Syndrome (HCPS). Methods. In this investigation, we report the expression of the N protein of the Araraquara Hantavirus in a Baculovirus Expression System, the use of this protein in IgM and IgG ELISA and comparison with the same antigen generated in E. coli. Results: The protein obtained, and purified in a nickel column, was effectively recognized by antibodies from confirmed HCPS patients. Comparison of the baculovirus generated antigen with the N protein produced in E. coli showed that both were equally effective in terms of sensitivity and specificity. Conclusions: Our results therefore indicate that either of these proteins can be used in serological tests in Brazil. © 2011 Machado et al; licensee BioMed Central Ltd.