Expression of recombinant Araraquara Hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in Escherichia coli

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dc.contributor.authorMacHado A.M.
dc.contributor.authorMacHado A.R.S.R.
dc.contributor.authorMoreli M.L.
dc.contributor.authorRibeiro B.M.
dc.contributor.authorFigueiredo L.T.M.
dc.contributor.authorWolff J.L.C.
dc.date.accessioned2024-03-13T01:11:18Z
dc.date.available2024-03-13T01:11:18Z
dc.date.issued2011
dc.description.abstractBackground: Antigens for Hantavirus serological tests have been produced using DNA recombinant technology for more than twenty years. Several different strategies have been used for that purpose. All of them avoid the risks and difficulties involved in multiplying Hantavirus in the laboratory. In Brazil, the Araraquara virus is one of the main causes of Hantavirus Cardio-Pulmonary Syndrome (HCPS). Methods. In this investigation, we report the expression of the N protein of the Araraquara Hantavirus in a Baculovirus Expression System, the use of this protein in IgM and IgG ELISA and comparison with the same antigen generated in E. coli. Results: The protein obtained, and purified in a nickel column, was effectively recognized by antibodies from confirmed HCPS patients. Comparison of the baculovirus generated antigen with the N protein produced in E. coli showed that both were equally effective in terms of sensitivity and specificity. Conclusions: Our results therefore indicate that either of these proteins can be used in serological tests in Brazil. © 2011 Machado et al; licensee BioMed Central Ltd.
dc.description.volume8
dc.identifier.doi10.1186/1743-422X-8-218
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36989
dc.relation.ispartofVirology Journal
dc.rightsAcesso Aberto
dc.titleExpression of recombinant Araraquara Hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in Escherichia coli
dc.typeArtigo
local.scopus.citations3
local.scopus.eid2-s2.0-79955775562
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955775562&origin=inward
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