An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

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dc.contributor.authorVan Oven C.
dc.contributor.authorKrawczyk P.M.
dc.contributor.authorStap J.
dc.contributor.authorMelo A.M.
dc.contributor.authorPiazzetta M.H.O.
dc.contributor.authorGobbi A.L.
dc.contributor.authorVan Veen H.A.
dc.contributor.authorVerhoeven J.
dc.contributor.authorAten J.A.
dc.date.accessioned2024-03-13T01:34:40Z
dc.date.available2024-03-13T01:34:40Z
dc.date.issued2009
dc.description.abstractLocalized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.
dc.description.firstpage721
dc.description.issuenumber6
dc.description.lastpage728
dc.description.volume38
dc.identifier.doi10.1007/s00249-009-0472-7
dc.identifier.issn0175-7571
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37359
dc.relation.ispartofEuropean Biophysics Journal
dc.rightsAcesso Aberto
dc.subject.otherlanguageDNA damage
dc.subject.otherlanguageDNA double-strand breaks
dc.subject.otherlanguageLive-cell microscopy
dc.subject.otherlanguageMicrobeam irradiation
dc.subject.otherlanguageSoft X-rays
dc.titleAn ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
dc.typeArtigo
local.scopus.citations14
local.scopus.eid2-s2.0-67651177417
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67651177417&origin=inward
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