An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

Van Oven C.; Krawczyk P.M.; Stap J.; Melo A.M.; Piazzetta M.H.O.; Gobbi A.L.; Van Veen H.A.; Verhoeven J.; Aten J.A.

An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

Tipo

Artigo

Data de publicação

2009

Periódico

European Biophysics Journal

Citações (Scopus)

14

Autores

Van Oven C.
Krawczyk P.M.
Stap J.
Melo A.M.
Piazzetta M.H.O.
Gobbi A.L.
Van Veen H.A.
Verhoeven J.
Aten J.A.

Resumo

Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.