Estudo experimental da inibição do VEGF na regeneração hepática

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dc.contributor.advisorMalafaia, Osvaldo
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/0300957415364593por
dc.contributor.authorSousa, Eros Luiz de
dc.creator.Latteshttp://lattes.cnpq.br/7564200346045204por
dc.date.accessioned2021-12-15T19:34:01Z
dc.date.available2021-12-15T19:34:01Z
dc.date.issued2019-01-09
dc.description.abstractBackground: extensive liver resections and transplants of liver parts are possible thanks to its regenerative capacity which is dependent on vascular endothelial growth factor (VEGF) activation which also regulates the angiogenesis. Bevacizumab (BV), a monoclonal antibody VEGF inhibitor, is a neoadjuvant treatment of cancers widely used, restricting microvascular density and may cause undesirable effects on postoperative healing and liver regeneration (LR). Objective: to determine the effect of a single dose (5mg/kg) of BV in LR. Methods: 32 rats submitted to partial hepatectomy (PH) were separated into two groups according to saline solution (C) or BV (BV) treatment. According to the observation time (48h and 15 days) they were subdivided in C48 and BV48 and C15 and BV15 sub-groups. In LR, the BV effects were evaluated by weight variation and liver function (albumin, transaminase, bilirubin and APRI ratio) and in remaining liver were evaluated, mitotic activity, microvascular density (angiogenesis), nuclear proliferation, histopathology for fibrosis, edema and congestion. For statistical analysis, the ANOVA and T-Student test was used (of statistical significance when p≤0.05). Results: on the fifteenth day after PH and BV treatment, there were no differences in weight evaluations (p=0.0691), liver function and mitotic indexes (p=0.1576). However, there was a decrease in cell viability (p=0.0000), microvascular density (p = 0.0162) and worsening in the occurrence of histopathological changes such as liver fibrosis, edema and congestion. Conclusion: a single BV dose, on the fifteenth day of evolution has no significant effect in weight evolution, serum biochemical evaluations (albumin, bilirubin and transaminases) and mitotic index. However, it induced microvascular density decreased, cell viability and liver histopathological changes such as fibrosis, edema and congestion.eng
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superiorpor
dc.formatapplication/pdf*
dc.identifier.citationSOUSA, Eros Luiz de. Estudo experimental da inibição do VEGF na regeneração hepática. 2019. 135 p. Tese( Princípios da Cirurgia) - Faculdade Evangélica Mackenzie do Paraná, Curitiba .por
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/28448
dc.keywordsLiver regenerationeng
dc.keywordsangiogenesis inhibitorseng
dc.keywordsbevacizumabeng
dc.keywordsVEGFeng
dc.keywordsrats, wistareng
dc.languageporpor
dc.publisherUniversidade Presbiteriana Mackenziepor
dc.rightsAcesso Abertopor
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectregeneração hepáticapor
dc.subjectinibidores da ingiogênesepor
dc.subjectbevacizumabpor
dc.subjectVEGFpor
dc.subjectratos wistarpor
dc.subject.cnpqCNPQ::CIENCIAS DA SAUDEpor
dc.titleEstudo experimental da inibição do VEGF na regeneração hepáticapor
dc.typeTesepor
local.contributor.board1Repka, João Carlos Domingues
local.contributor.board1Lattes9033132753691332por
local.contributor.board2Torres, Maria Fernanda Pioli
local.contributor.board2Latteshttp://lattes.cnpq.br/4570739681544375por
local.contributor.board3Nicoluzzi, João Eduardo Leal
local.contributor.board3Latteshttp://lattes.cnpq.br/6966261370146302por
local.contributor.board4Nassif, Paulo Afonso Nunes
local.contributor.board4Latteshttp://lattes.cnpq.br/5902051031307945por
local.publisher.countryBrasilpor
local.publisher.departmentFaculdade Evangélica Mackenzie do Paraná (FEMPAR)por
local.publisher.initialsUPMpor
local.publisher.programPrincípios da Cirurgiapor
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Princípios da Cirurgia - Teses - Medicina Curitiba