Análise histopatológica de articulações com osteoartrite tratada com diferentes medicamentos intra-articulares: estudo experimental em ratos
Tipo
TCC
Data de publicação
2025-05-29
Periódico
Citações (Scopus)
Autores
Gomes, Heloisa Henriques
Okimura, Vitória Naomi
Okimura, Vitória Naomi
Orientador
Mehanna, Samya Hamad
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
Introdução: A osteoartrite, também nomeada doença articular degenerativa, é a forma mais comum de artrite no mundo, afetando principalmente a população idosa. Com o envelhecimento global, essa condição tem se tornado uma importante causa de incapacidade física e afastamento do trabalho, resultando em custos significativos para o sistema de saúde e previdência. Objetivo: Avaliar histopatologicamente e comparar o efeito condro regenerador e anti inflamatório do Plasma Rico em Plaquetas (PRP), Ácido Hialurônico, Triancinolona e Sulfato de Condroitina em um modelo experimental de osteoartrite induzida por Zymosan em ratos. Materiais e Métodos: Foram utilizados 30 ratos machos Wistar, divididos em cinco grupos. A osteoartrite foi induzida pela injeção de Zymosan® na articulação femoro-tibio-patelar direita. Cada grupo recebeu um dos tratamentos testados por via intra-articular a cada 21 dias, totalizando 60 dias de intervenção. Um grupo adicional foi mantido sem tratamento como controle. Radiografias e ressonâncias magnéticas foram realizadas no 30º e 90º dia para confirmar a indução da osteoartrite e avaliar a eficácia dos tratamentos. No 60º dia, os animais foram sacrificados para análise histopatológica das articulações. Resultados: O grupo tratado com PRP apresentou maior quantidade de condrócitos colunares maduros e organizados, evidenciando um tratamento eficaz para a regeneração articular. O HA demonstrou poucas erosões e úlceras superficiais na cartilagem, com alta quantidade de condrócitos por campo, indicando condroproteção e regeneração articular. Conclusão: O PRP mostrou um efeito condrorregenerador superior em comparação ao Ácido Hialurônico, Triancinolona e Condroitina. No entanto, o Ácido Hialurônico foi mais eficaz na redução da inflamação.
Introduction: Osteoarthritis, also known as degenerative joint disease, is the most common form of arthritis worldwide, primarily affecting the elderly population. With global aging, this condition has become a major cause of physical disability and work absenteeism, resulting in significant costs for healthcare and social security systems. Objective: To histopathologically evaluate and compare the chondroregenerative and anti-inflammatory effects of Platelet-Rich Plasma (PRP), Hyaluronic Acid (HA), Triamcinolone, and Chondroitin Sulfate in an experimental model of Zymosan-induced osteoarthritis in rats. Materials and Methods: Thirty male Wistar rats were used and divided into five groups. Osteoarthritis was induced by intra-articular injection of Zymosan® into the right femorotibial-patellar joint. Each group received one of the tested treatments intra-articularly every 21 days, totaling 60 days of intervention. An additional group was maintained without treatment as a control. Radiographs and magnetic resonance imaging were performed on days 30 and 90 to confirm osteoarthritis induction and assess treatment efficacy. On day 60, the animals were euthanized for histopathological analysis of the joints. Results: The group treated with PRP showed a greater number of mature, organized columnar chondrocytes, indicating effective joint regeneration. HA treatment showed few erosions and superficial cartilage ulcers, with a high number of chondrocytes per field, suggesting chondroprotection and joint regeneration. Conclusion: PRP demonstrated superior chondroregenerative effects compared to Hyaluronic Acid, Triamcinolone, and Chondroitin. However, Hyaluronic Acid was more effective in reducing inflammation.
Introduction: Osteoarthritis, also known as degenerative joint disease, is the most common form of arthritis worldwide, primarily affecting the elderly population. With global aging, this condition has become a major cause of physical disability and work absenteeism, resulting in significant costs for healthcare and social security systems. Objective: To histopathologically evaluate and compare the chondroregenerative and anti-inflammatory effects of Platelet-Rich Plasma (PRP), Hyaluronic Acid (HA), Triamcinolone, and Chondroitin Sulfate in an experimental model of Zymosan-induced osteoarthritis in rats. Materials and Methods: Thirty male Wistar rats were used and divided into five groups. Osteoarthritis was induced by intra-articular injection of Zymosan® into the right femorotibial-patellar joint. Each group received one of the tested treatments intra-articularly every 21 days, totaling 60 days of intervention. An additional group was maintained without treatment as a control. Radiographs and magnetic resonance imaging were performed on days 30 and 90 to confirm osteoarthritis induction and assess treatment efficacy. On day 60, the animals were euthanized for histopathological analysis of the joints. Results: The group treated with PRP showed a greater number of mature, organized columnar chondrocytes, indicating effective joint regeneration. HA treatment showed few erosions and superficial cartilage ulcers, with a high number of chondrocytes per field, suggesting chondroprotection and joint regeneration. Conclusion: PRP demonstrated superior chondroregenerative effects compared to Hyaluronic Acid, Triamcinolone, and Chondroitin. However, Hyaluronic Acid was more effective in reducing inflammation.
Descrição
Palavras-chave
osteoartrite , histologia , medicamentos , osteoarthritis , histology , pharmaceutical preparations