Expression of Parkin, APC, APE1, and Bcl-xL in Colorectal Polyps
Tipo
Artigo
Data de publicação
2021
Periódico
Journal of Histochemistry and Cytochemistry
Citações (Scopus)
1
Autores
Kuhl Svoboda Baldin R.
Australia Paredes Marcondes Ribas C.
de Noronha L.
Veloso da Silva-Camargo C.C.
Santos Sotomaior V.
Martins Sebastiao A.P.
Vasconcelos de Castilho A.P.
Rodrigues Montemor Netto M.
Australia Paredes Marcondes Ribas C.
de Noronha L.
Veloso da Silva-Camargo C.C.
Santos Sotomaior V.
Martins Sebastiao A.P.
Vasconcelos de Castilho A.P.
Rodrigues Montemor Netto M.
Orientador
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
© The Author(s) 2021.Colorectal cancer can develop through molecular, chromosomal, and epigenetic cumulative changes that transform the normal intestinal epithelium into the colorectal polyps, called conventional adenomas (CAs) or serrated polyps (SPs), recognized as precursors of invasive colorectal neoplasia. These benign lesions need to explore the morphology, histological diagnosis, and biomarkers profile to accurately characterize lesions with potential for evolution to cancer. This study aimed to correlate the immunohistochemical expression of Parkin and Adenomatous Polyposis Coli (APC; tumor suppressors), Human Apurinic/Apyrimidinic endonuclease 1 (APE1), and B-cell lymphoma-extra-large (Bcl-xL; oncogenic proteins) in sporadic colorectal polyps with clinical, endoscopic, and diagnostic data. Immunohistochemical analysis was performed on tissue microarray samples of 306 polyps. Based on the Allred score, the expressions were graduated in the cytoplasm and nucleus of superficial and cryptic cells. There was higher Parkin nuclear expression (p=0.006 and 0.010) and APC cytoplasmic expression in cryptic cells (p<0.001) in SPs. CAs, APE1 (p<0.001) and Bcl-xL (p<0.001) were more expressed in the nuclei and cytoplasms, respectively. These results are related to the biological role proposed for these proteins in cellular functions. They can contribute to the diagnosis criteria for polyps and improve the knowledge of biomarkers that could predict cancer development:
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Assuntos Scopus
Adenomatous Polyposis Coli Protein , bcl-X Protein , Colonic Polyps , DNA-(Apurinic or Apyrimidinic Site) Lyase , Gene Expression Regulation , Humans , Ubiquitin-Protein Ligases