Oxidized multiwalled carbon nanotubes as antigen delivery system to promote superior CD8+ T Cell response and protection against Cancer

Faria P.C.B.D.; Santos L.I.D.; Coelho J.P.; Ribeiro H.B.; Pimenta M.A.; Ladeira L.O.; Gomes D.A.; Furtado C.A.; Gazzinelli R.T.

Oxidized multiwalled carbon nanotubes as antigen delivery system to promote superior CD8+ T Cell response and protection against Cancer

Tipo

Artigo

Data de publicação

2014

Periódico

Nano Letters

Citações (Scopus)

93

Autores

Faria P.C.B.D.
Santos L.I.D.
Coelho J.P.
Ribeiro H.B.
Pimenta M.A.
Ladeira L.O.
Gomes D.A.
Furtado C.A.
Gazzinelli R.T.

Resumo

© 2014 American Chemical Society.Properties like high interfacial area with cellular membranes, unique ability to incorporate multiple functionalization, as well as compatibility and transport in biological fluids make carbon nanotubes (CNTs) useful for a variety of therapeutic and drug-delivery applications. Here we used a totally synthetic hybrid supramolecule as an anticancer vaccine formulation. This complex structure comprises CNTs as delivery system for the Cancer Testis Antigen named NY-ESO-1, allied to a synthetic Toll-Like Receptor agonist. The CNT constructs were rapidly internalized into dendritic cells, both in vitro and in vivo, and served as an intracellular antigen depot. This property favored the induction of strong CD4+ T as well as CD8+ T cell-mediated immune responses against the NY-ESO-1. Importantly, the vaccination significantly delayed the tumor development and prolonged the mice survival, highlighting the potential application of CNTs as a vaccine delivery system to provide superior immunogenicity and strong protection against cancer.

Assuntos Scopus

Antigen delivery system , cancer , Cell-mediated immune , Multiple functionalization , Nanovaccine , NY-ESO-1 , Toll-like receptors , Vaccine delivery system , Animals , Anticarcinogenic Agents , Antigens , Antigens, Neoplasm , Calibration , Cancer Vaccines , CD4-Positive T-Lymphocytes , CD8-Positive T-Lymphocytes , Cell Proliferation , CpG Islands , Dendritic Cells , Female , Flow Cytometry , Humans , Lymphocytes , Membrane Proteins , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Microscopy, Electron, Transmission , Nanotechnology , Nanotubes, Carbon , Neoplasms , Oxygen , Peptides , Spectrum Analysis, Raman