Validação de modelo animal de depressão induzida por deficiência de vitamina B12
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Arquivos
Tipo
Dissertação
Data de publicação
2017-08-24
Periódico
Citações (Scopus)
Autores
Brito, Adriana Maria de Oliveira
Orientador
Cysneiros, Roberta Monterazzo
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Pereira , Isabela Rosier Olímpio
Silva, Sérgio Gomes da
Silva, Sérgio Gomes da
Programa
Distúrbios do Desenvolvimento
Resumo
Depression affects 13% to 20% of the world's population. Vitamin B12 deficiency is related to
depression. The lack of this vitamin can be induced in rats by the addition of pectin to the
diet. This depletion generates aberrations in base substitutions and DNA methylation. Given
the need for animal models with neurobiological fundamentals that best represent
depression, the objective of this paper was to validate and characterize an experimental
model of depression induced by vitamin B12 deficiency in female rats fed with an added diet
pectin. The project was elaborated in two phases and involved 40 Wistar rats, monitored
from their weaning to their 133th day of life. In the first phase, the animals were randomly
separated into two groups, which differed by the feed offered (ration with or without pectin).
At the end of this phase, the animals were submitted to a blood count and tests of positive
contrast of sucrose, open field and high cross labyrinth, to evaluate if the vitamin deficiency
had altered hematological and behavioral variables.
In the second phase, the pectin group was divided into three groups: one group continued
with the same diet, another group continued with the same diet but also received
antidepressant in the water and a third group started to be fed with the regular feed. The
pectin-free fed group was divided into two groups: one continued with the same feed and the
other fed with increased pectin feed.
At the end of this phase the animals were submitted to the same tests listed above and,
after their euthanasia, the brains were removed for analysis of the expression of genes
involved in depression in rats in the hippocampus and prefrontal cortex. The dropout times
obtained by the groups in the forced swim test (pectin = 108 seconds and standard group =
77 seconds, with p = 0.03), applied at the end of the first phase, indicate that the pectin-fed
group was behavior-induced of the depressive type.
The output of the other tests confirm that this result did not occur due to locomotor and/ or
anxiogenic problems. In the second phase, there was no difference between the times, which
indicates that this behavior was not observed at this time of the lives of the animals. It is
concluded, then, that pectin intake can induces rats to depression but possibly only within a
time window that goes until 11 weeks of age. There are three other hypotheses that may
justify the results found: there was a physiological adaptation to pectin consumption; older
animals may require increased fiber intake to decrease vitamin uptake or perhaps more
intense exhaustion at this stage of life is required to lead to this behavior.
Descrição
Palavras-chave
vitamina B12 , depressão , pectina , teste do nado forçado
Assuntos Scopus
Citação
BRITO, Adriana Maria de Oliveira. Validação de modelo animal de depressão induzida por deficiência de vitamina B12. 2017. 44 f . Dissertação( Distúrbios do Desenvolvimento) - Universidade Presbiteriana Mackenzie, São Paulo .