Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells

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dc.contributor.authorCarneiro Z.A.
dc.contributor.authorBiazzotto J.C.
dc.contributor.authorAlexiou A.D.P.
dc.contributor.authorNikolaou S.
dc.date.accessioned2024-03-13T01:00:36Z
dc.date.available2024-03-13T01:00:36Z
dc.date.issued2014
dc.description.abstractIn vitro cytotoxicity study of the [Ru3O(CH3COO) 6(4-pic)2(NO)]PF6 triruthenium nitrosyl cluster (compound 1, 4-pic = 4-methylpyridine) against B16F10 melanoma cell line was evaluated in the presence and absence of visible light irradiation. The nitrosyl cluster 1 showed a significant tumoricidal activity when irradiated at λ = 532 nm, reducing cell viability up to 90% at a concentration of 62.5 μM. However, cell death of 60% is also observed in the dark which can be assigned to the NO release mediated by a redox reaction of the cluster in cell medium. This possibility was confirmed by amperometric detection of NO after the addition of ascorbic acid to compound 1 in phosphate buffer. A control experiment was performed with the solvated cluster [Ru3O(CH3COO) 6(4-pic)2(CH3OH)]PF6 (compound 2) and no significant lowering of cell viability was observed. These results suggest that the nitrosyl cluster acts as a pro-drug, delivering NO, which is the actual active species. © 2014 Elsevier Inc.
dc.description.firstpage36
dc.description.lastpage38
dc.description.volume134
dc.identifier.doi10.1016/j.jinorgbio.2014.01.012
dc.identifier.issn0162-0134
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36388
dc.relation.ispartofJournal of Inorganic Biochemistry
dc.rightsAcesso Restrito
dc.subject.otherlanguageMelanoma
dc.subject.otherlanguageNitric oxide release
dc.subject.otherlanguageTriruthenium cluster
dc.subject.otherlanguageVisible light irradiation
dc.titleNitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells
dc.typeArtigo
local.scopus.citations36
local.scopus.eid2-s2.0-84893835069
local.scopus.subjectAnimals
local.scopus.subjectAntineoplastic Agents
local.scopus.subjectCell Line, Tumor
local.scopus.subjectCell Survival
local.scopus.subjectCoordination Complexes
local.scopus.subjectLight
local.scopus.subjectMice
local.scopus.subjectNitric Oxide
local.scopus.subjectNitric Oxide Donors
local.scopus.subjectOxidation-Reduction
local.scopus.subjectPhotolysis
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893835069&origin=inward
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