Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells
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Artigo
Date
2014
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Journal of Inorganic Biochemistry
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36
Authors
Carneiro Z.A.
Biazzotto J.C.
Alexiou A.D.P.
Nikolaou S.
Biazzotto J.C.
Alexiou A.D.P.
Nikolaou S.
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Volume Title
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Abstract
In vitro cytotoxicity study of the [Ru3O(CH3COO) 6(4-pic)2(NO)]PF6 triruthenium nitrosyl cluster (compound 1, 4-pic = 4-methylpyridine) against B16F10 melanoma cell line was evaluated in the presence and absence of visible light irradiation. The nitrosyl cluster 1 showed a significant tumoricidal activity when irradiated at λ = 532 nm, reducing cell viability up to 90% at a concentration of 62.5 μM. However, cell death of 60% is also observed in the dark which can be assigned to the NO release mediated by a redox reaction of the cluster in cell medium. This possibility was confirmed by amperometric detection of NO after the addition of ascorbic acid to compound 1 in phosphate buffer. A control experiment was performed with the solvated cluster [Ru3O(CH3COO) 6(4-pic)2(CH3OH)]PF6 (compound 2) and no significant lowering of cell viability was observed. These results suggest that the nitrosyl cluster acts as a pro-drug, delivering NO, which is the actual active species. © 2014 Elsevier Inc.
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Keywords
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Animals , Antineoplastic Agents , Cell Line, Tumor , Cell Survival , Coordination Complexes , Light , Mice , Nitric Oxide , Nitric Oxide Donors , Oxidation-Reduction , Photolysis