Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells
Tipo
Artigo
Data de publicação
2014
Periódico
Journal of Inorganic Biochemistry
Citações (Scopus)
36
Autores
Carneiro Z.A.
Biazzotto J.C.
Alexiou A.D.P.
Nikolaou S.
Biazzotto J.C.
Alexiou A.D.P.
Nikolaou S.
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Título da Revista
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Título de Volume
Membros da banca
Programa
Resumo
In vitro cytotoxicity study of the [Ru3O(CH3COO) 6(4-pic)2(NO)]PF6 triruthenium nitrosyl cluster (compound 1, 4-pic = 4-methylpyridine) against B16F10 melanoma cell line was evaluated in the presence and absence of visible light irradiation. The nitrosyl cluster 1 showed a significant tumoricidal activity when irradiated at λ = 532 nm, reducing cell viability up to 90% at a concentration of 62.5 μM. However, cell death of 60% is also observed in the dark which can be assigned to the NO release mediated by a redox reaction of the cluster in cell medium. This possibility was confirmed by amperometric detection of NO after the addition of ascorbic acid to compound 1 in phosphate buffer. A control experiment was performed with the solvated cluster [Ru3O(CH3COO) 6(4-pic)2(CH3OH)]PF6 (compound 2) and no significant lowering of cell viability was observed. These results suggest that the nitrosyl cluster acts as a pro-drug, delivering NO, which is the actual active species. © 2014 Elsevier Inc.
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Assuntos Scopus
Animals , Antineoplastic Agents , Cell Line, Tumor , Cell Survival , Coordination Complexes , Light , Mice , Nitric Oxide , Nitric Oxide Donors , Oxidation-Reduction , Photolysis