Is the FVB/N mouse strain truly resistant to diet-induced obesity?

Nascimento-Sales M.; Fredo-da-Costa I.; Borges Mendes A.C.B.; Melo S.; Ravache T.T.; Gomez T.G.B.; Gaisler-Silva F.; Ribeiro M.O.; Santos A.R.; Carneiro-Ramos M.S.; Christoffolete M.A.

Is the FVB/N mouse strain truly resistant to diet-induced obesity?

Tipo

Artigo

Data de publicação

2017

Periódico

Physiological Reports

Citações (Scopus)

21

Autores

Nascimento-Sales M.
Fredo-da-Costa I.
Borges Mendes A.C.B.
Melo S.
Ravache T.T.
Gomez T.G.B.
Gaisler-Silva F.
Ribeiro M.O.
Santos A.R.
Carneiro-Ramos M.S.
Christoffolete M.A.

Resumo

© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.C57Bl/6J mice are the gold standard animal model of diet-induced obesity. These animals become obese with higher adiposity, blood fasting glucose, triglycerides, and total cholesterol when fed a high-fat diet (HFD). Conversely, the FVB/N mouse line is thought to be resistant to diet-induced obesity, with low or no weight gain and adiposity in response to a HFD. In this study, we investigated whether FVB/N mice are resistant or susceptible to metabolic disorder that is promoted by a HFD. Biometric parameters and blood chemistry were analyzed in C57Bl/6J and FVB/N mice that were fed a chow diet or HFD. Glucose and insulin sensitivity were assessed by performing the glucose tolerance test and measuring serum insulin/glucose and homeostasis model assessment-insulin resistance. Metabolism-related gene expression was investigated by real-time reverse transcription polymerase chain reaction. Adipocyte morphology and liver steatosis were evaluated using standard histology. FVB/N mice had higher adiposity than C57Bl/6J mice that were fed a chow diet and were glucose intolerant. FVB/N mice that were fed a HFD presented higher insulin resistance and greater liver steatosis. Epididymal white adipose tissue exhibited severe inflammation in FVB/N mice that were fed a HFD. The FVB/N mouse strain is suitable for studies of diet-induced obesity, and the apparent lack of a HFD-induced response may reveal several strain-specific events that are triggered by a HFD. Further studies of the FVB/N background may shed light on the complex multifactorial symptoms of obesity and metabolic syndrome.

Assuntos Scopus

Adiposity , Animals , Blood Glucose , Diet, High-Fat , Disease Models, Animal , Genetic Background , Male , Mice , Mice, Inbred C57BL , Mice, Obese , Obesity