Response of leucine metabolism to hyperinsulinemia in hypothyroid patients before and after thyroxine replacement

Rochon C.; Tauveron I.; Dejax C.; Benoit P.; Capitan P.; Bayle G.; Prugnaud J.; Fabricio A.; Berry C.; Champredon C.; Thieblot P.; Grizard J.

Response of leucine metabolism to hyperinsulinemia in hypothyroid patients before and after thyroxine replacement

Tipo

Artigo

Data de publicação

2000

Periódico

Journal of Clinical Endocrinology and Metabolism

Citações (Scopus)

13

Autores

Rochon C.
Tauveron I.
Dejax C.
Benoit P.
Capitan P.
Bayle G.
Prugnaud J.
Fabricio A.
Berry C.
Champredon C.
Thieblot P.
Grizard J.

Resumo

We have investigated the effect of hypothyroidism and insulin on protein metabolism in humans. Six hypothyroid patients were studied in a postabsorptive state before and after 5 months of regular treatment for hypothyroidism (153 ± 17 μg/day of L-T4). The effect of insulin was assessed under hyperinsulinemic euglycemic and eukalemic conditions. Insulin was infused for 140 min at 0.0063 ± 0.0002 nmol/kg·min. An amino acid infusion was used to blunt insulin-induced hypoaminoacidemia. Whole body protein turnover was measured using L-[1-3C] leucine. When compared to L-T4-induced subclinical thyrotoxic state, hypothyroidism induced a significant decrease (P < 0.05) in leucine endogenous appearance rate (a reflection of proteolysis; 0.89 ± 0.09 vs. 1.33 ± 0.05 μmol/kg·min), oxidation (0.19 ± 0.02 vs. 0.25 ± 0.03 μmol/kg·min), and nonoxidative disposal (a reflection of protein synthesis; 0.87 ± 0.11 vs. 1.30 ± 0.05 μmol/kg·min). Insulin lowered proteolysis during both the subclinical thyrotoxic and hypothyroid states. Hypothyroidism impaired the antiproteolytic effects of insulin. Thyroid hormones are, therefore, essential for the normal antiproteolytic action of insulin.