Gold nanoparticles coupled with graphene quantum dots in organized medium to quantify aminoglycoside anti-biotics in yellow fever vaccine after solid phase extraction using a selective imprinted polymer

Data de publicação
Journal of Pharmaceutical and Biomedical Analysis
Citações (Scopus)
Toloza C.A.T.
Almeida J.M.S.
Khan S.
dos Santos Y.G.
da Silva A.R.
Romani E.C.
Larrude D.G.
Freire F.L.
Aucelio R.Q.
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© 2018 Elsevier B.V.The determination of kanamycin sulfate was made indirectly by measuring its effect on photoluminescent amino functionalized graphene quantum dots (GQDs-amino) associated with gold nanoparticles (AuNPs) that were produced by the reduction of AuCl4 with NaBH4 in an aqueous dispersion of GQDs-amino (obtained by the pyrolysis of citric acid and glutathione) also containing the cationic surfactant CTAB. The AuNPs-GQDs-amino-CTAB system presents a suppressed photoluminescence that is amplified in the presence of kanamycin. Under optimized experimental conditions, the photoluminescence amplification of the nanomaterial system showed a linear response as a function of kanamycin concentration, covering three orders of magnitude (10−7 to 10−5 mol L−1). The use of solid phase extraction with a cartridge packed with aminoglycoside selective molecularly imprinted polymer ensured selectivity in determinations made on yellow-fever vaccine and veterinary pharmaceutical formulations. The analytical results were statistically similar to those obtained with an HPLC-based fluorescence method (after chemical derivatization). The proposed method is a simple, sensitive and selective approach that does not involve the use of toxic reagents employed for chemical derivatization of aminoglycoside antibiotics.
Assuntos Scopus
Anti-Bacterial Agents , Biosensing Techniques , Gold , Graphite , Kanamycin , Metal Nanoparticles , Polymers , Preservatives, Pharmaceutical , Quantum Dots , Sensitivity and Specificity , Solid Phase Extraction , Yellow Fever Vaccine
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