Identificação da microbiota vaginal de mulheres atendidas em consultas ginecológicas de rotina
Tipo
TCC
Data de publicação
2024-06-20
Periódico
Citações (Scopus)
Autores
Morais, Felipe Mota Narciso de
Moreira, Luan Carlos Carvalho
Moreira, Luan Carlos Carvalho
Orientador
Wormsbecker, Liya Regina Mikami
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ISSN da Revista
Título de Volume
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Resumo
Introdução: estudos de caracterização da microbiota vaginal por meio de sequenciamento genético aprimoraram muito a compreensão desse ecossistema. Contudo, evidências de variação da microbiota entre diferentes populações destacam a importância de uma caracterização microbiológica contextualizada, considerando parâmetros sociodemográficos, assim como fatores fisiológicos próprios da saúde feminina. Objetivos: caracterizar a microbiota bacteriana vaginal de mulheres atendidas no Ambulatório Acadêmico da Faculdade Evangélica Mackenzie do Paraná (FEMPAR), verificar se há correlação entre os CST e parâmetros sociodemográficos, faixas etárias e fase reprodutiva. Métodos: O estudo foi transversal, observacional e descritivo, realizado com 22 mulheres, com idades entre 18 e 69 anos, atendidas pelo serviço de Ginecologia do Ambulatório Acadêmico da FEMPAR. Realizou-se a coleta do material biológico e a aplicação de um questionário em consultas de rotina entre 3 de outubro de 2023 e 20 de fevereiro de 2024. As amostras foram, então, processadas e analisadas mediante sequenciamento genético de nova geração e agrupadas conforme fase reprodutiva e faixas etárias para estudo. O Teste Exato de Fisher foi aplicado para testar as correlações. Resultados: No total, 22 pacientes foram estudadas, com média de idade de 40,4 anos. A maioria das mulheres tinha IMC normal (36,4%), eram eurodescendentes (54,5%) e não fumavam (90,9%). Em termos de saúde reprodutiva, 77,2% estavam em menacme e 54,5% não usavam métodos contraceptivos. Na análise, Lactobacillus foi o gênero mais prevalente, especialmente L. iners (22,95%). Não houve correlações significativas entre as comunidades microbiológicas (CST) e os parâmetros sociodemográficos ou fases reprodutivas. Nenhuma das correlações testadas foi estatisticamente significativa nessa amostra. Conclusão: O emprego de técnicas modernas de sequenciamento genético permite estudar detalhadamente a microbiota vaginal possibilitando não apenas melhores perspectivas para futuras condutas médicas, mas também elucidar as complexidades desse ecossistema e seu impacto na saúde feminina.
Introduction: studies characterizing the vaginal microbiota through genetic sequencing have greatly improved our understanding of this ecosystem. However, evidence of microbiota variation between different populations highlights the importance of contextualized microbiological characterization, taking into account sociodemographic parameters as well as physiological factors specific to women's health. Objectives: To characterize the vaginal bacterial microbiota of women seen at the Academic Outpatient Clinic of the Mackenzie Evangelical College of Paraná (FEMPAR), and to see if there is a correlation between TSC and sociodemographic parameters, age groups and reproductive stage. Methods: This was a cross-sectional, observational and descriptive study carried out with 22 women aged between 18 and 69 who were seen by the Gynecology service at the FEMPAR Academic Outpatient Clinic. Biological material was collected and a questionnaire was administered during routine consultations between October 3, 2023 and February 20, 2024. The samples were then processed and analyzed using next-generation genetic sequencing and grouped according to reproductive stage and age group for study. Fisher's Exact Test was applied to test for correlations. Results: A total of 22 patients were studied, with a mean age of 40.4 years. Most of the women had a normal BMI (36.4%), were of European descent (54.5%) and did not smoke (90.9%). In terms of reproductive health, 77.2% were in menopause and 54.5% were not using contraception. In the analysis, Lactobacillus was the most prevalent genus, especially L. iners (22.95%). There were no significant correlations between microbiological communities (CST) and sociodemographic parameters or reproductive stages. None of the correlations tested were statistically significant in this sample. Conclusion: The use of modern genetic sequencing techniques makes it possible to study the vaginal microbiota in detail, enabling not only better prospects for future medical approaches, but also to elucidate the complexities of this ecosystem and its impact on women's health.
Introduction: studies characterizing the vaginal microbiota through genetic sequencing have greatly improved our understanding of this ecosystem. However, evidence of microbiota variation between different populations highlights the importance of contextualized microbiological characterization, taking into account sociodemographic parameters as well as physiological factors specific to women's health. Objectives: To characterize the vaginal bacterial microbiota of women seen at the Academic Outpatient Clinic of the Mackenzie Evangelical College of Paraná (FEMPAR), and to see if there is a correlation between TSC and sociodemographic parameters, age groups and reproductive stage. Methods: This was a cross-sectional, observational and descriptive study carried out with 22 women aged between 18 and 69 who were seen by the Gynecology service at the FEMPAR Academic Outpatient Clinic. Biological material was collected and a questionnaire was administered during routine consultations between October 3, 2023 and February 20, 2024. The samples were then processed and analyzed using next-generation genetic sequencing and grouped according to reproductive stage and age group for study. Fisher's Exact Test was applied to test for correlations. Results: A total of 22 patients were studied, with a mean age of 40.4 years. Most of the women had a normal BMI (36.4%), were of European descent (54.5%) and did not smoke (90.9%). In terms of reproductive health, 77.2% were in menopause and 54.5% were not using contraception. In the analysis, Lactobacillus was the most prevalent genus, especially L. iners (22.95%). There were no significant correlations between microbiological communities (CST) and sociodemographic parameters or reproductive stages. None of the correlations tested were statistically significant in this sample. Conclusion: The use of modern genetic sequencing techniques makes it possible to study the vaginal microbiota in detail, enabling not only better prospects for future medical approaches, but also to elucidate the complexities of this ecosystem and its impact on women's health.
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Palavras-chave
bactérias , composição da comunidade microbiana , RNA Ribossômico 16S , vagina , bacteria , microbiota , RNA , Ribosomal , 16S , vagina