Bifunctional fluorescent benzimidazo[1,2-α]quinolines for Candida spp. biofilm detection and biocidal activity

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dc.contributor.authorde Souza I.O.P.
dc.contributor.authorSchrekker C.M.L.
dc.contributor.authorLopes W.
dc.contributor.authorOrru R.V.A.
dc.contributor.authorHranjec M.
dc.contributor.authorPerin N.
dc.contributor.authorMachado M.
dc.contributor.authorOliveira L.F.
dc.contributor.authorDonato R.K.
dc.contributor.authorStefani V.
dc.contributor.authorFuentefria A.M.
dc.contributor.authorSchrekker H.S.
dc.date.accessioned2024-03-13T00:52:19Z
dc.date.available2024-03-13T00:52:19Z
dc.date.issued2016
dc.description.abstract© 2016 Elsevier B.V.Biofilms provide an ideal environment for protecting the microbial cells from damage caused by humoral and cellular immune system components, promoting resistance, infections and increasing mortality and morbidity of patients in health facilities. In an attempt to provide an innovative solution for preventing contamination in hospital environments, this study evaluated nine structural complementary fluorescent benzimidazo[1,2-α]quinolines as bifunctional agents that both detect and have biocidal activity against yeast biofilms on stainless steel surfaces. The benzimidazoles' staining capability was determined by a fluorescence microscopy study and spraying the substance on yeast biofilm contaminated stainless steel surfaces. Furthermore, their in vitro human leukocyte cytotoxicity was evaluated with trypan blue and their biocidal activity was determined as the minimum inhibitory concentration against Candida tropicalis, C. albicans and C. parapsilosis strains. Moreover, scanning electron micrographs were recorded to study the biocidal activity. This resulted in the identification of 7, which presents all the desired characteristics (such as solubility) and capabilities (staining and biocide activity against all tested biofilm forming yeast strains) at the same time. As such, benzimidazole 7 has the potential to guarantee the use of disinfected medical and surgical instruments in clinical and surgical procedures, consequently, contributing to an increased safety for patients.
dc.description.firstpage319
dc.description.lastpage326
dc.description.volume163
dc.identifier.doi10.1016/j.jphotobiol.2016.08.037
dc.identifier.issn1873-2682
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/35923
dc.relation.ispartofJournal of Photochemistry and Photobiology B: Biology
dc.rightsAcesso Restrito
dc.subject.otherlanguageBiological probes
dc.subject.otherlanguageFungal biomarkers
dc.subject.otherlanguageLeukocyte cytotoxicity
dc.subject.otherlanguageStainless steel
dc.subject.otherlanguageYeast
dc.titleBifunctional fluorescent benzimidazo[1,2-α]quinolines for Candida spp. biofilm detection and biocidal activity
dc.typeArtigo
local.scopus.citations6
local.scopus.eid2-s2.0-84985961639
local.scopus.subjectAntifungal Agents
local.scopus.subjectBiofilms
local.scopus.subjectCandida albicans
local.scopus.subjectDrug Resistance, Fungal
local.scopus.subjectFluorescent Dyes
local.scopus.subjectMicrobial Sensitivity Tests
local.scopus.subjectQuinolines
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84985961639&origin=inward
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