Serum cytokine levels and acute graft-versus-host disease after HLA-identical hematopoietic stem cell transplantation

Visentainer J.E.L.; Lieber S.R.; Persoli L.B.L.; Vigorito A.C.; Aranha F.J.P.; De Brito Eid K.A.; Oliveira G.B.; Miranda E.C.M.; De Souza C.A.

Serum cytokine levels and acute graft-versus-host disease after HLA-identical hematopoietic stem cell transplantation

Tipo

Artigo

Data de publicação

2003

Periódico

Experimental Hematology

Citações (Scopus)

83

Autores

Visentainer J.E.L.
Lieber S.R.
Persoli L.B.L.
Vigorito A.C.
Aranha F.J.P.
De Brito Eid K.A.
Oliveira G.B.
Miranda E.C.M.
De Souza C.A.

Resumo

Objective. The aim of this study was to investigate whether the serum levels of soluble interleukin-2R (sIL-2R), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-6, IL-10, and transforming growth factor-β1 (TGF-β1) were associated with the development of acute graft-vs-host disease (aGVHD). Patients and Methods. Serum cytokine levels were sequentially measured by sandwich enzyme-linked immunosorbent assay in 13 patients who had received full-match allogeneic hematopoietic stem cell transplantation (HSCT). Results. Serum sIL-2R and IL-10 levels from the 1st to the 15th week post transplantation were significantly higher in the group that developed aGVHD than in the group without aGVHD. sIL-2R levels increased in direct correlation to engraftment and at onset of aGVHD, whereas IL-10 levels increased transiently following HSCT. The mean TNF-α concentration in the first weeks after transplantation was augmented in the group that developed aGVHD. Furthermore, a decrease in TGF-β1 levels after engraftment was significantly associated with aGVHD. No correlation was found between aGVHD and the other cytokines. Conclusions. These results support the idea that a balance between cytokines derived from type 1 and type 2 T-helper cells may be important in the development and control of aGVHD. Although sIL-2R, TNF-α, IL-10, and TGF-β1 levels have been correlated with aGVHD, sIL-2R levels at engraftment may provide a better parameter for early detection of aGVHD after allogeneic HSCT. © 2003 International Society for Experimental Hematology. Published by Elsevier Inc.