Quantification of neomycin in rubella vaccine by off/on metal ion mediated photoluminescence from functionalized graphene quantum dots

Data de publicação
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Citações (Scopus)
Pinto I.A.
Toloza C.A.T.
Almeida J.M.S.
da Silva A.R.
Larrude D.G.
Aucelio R.Q.
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
© 2019 Elsevier B.V.The determination of neomycin sulfate was made using photoluminescent amino-functionalized graphene quantum dots (obtained from hydro-exfoliation of a mixture of citric acid and glutathione). From the several ions tested, Fe3+ was the best mediator to enable an off/on photoluminescence effect used for quantification. The mediation of Fe3+ was found to be crucial as it is responsible for the photoluminescence quenching effect, due to the interaction with quantum dots surface, also having large affinity towards neomycin that removes Fe3+ from the surface of GQDs, consequently, promoting restoration of the original nanomaterial photoluminescence. Such signal restoration was proportional to the neomycin sulfate concentration added. The linearized analytical response covered three orders of magnitude (10−7 to 10−5 mol L−1). The proposed method is an alternative to those requiring labor-intensive procedures for chemical the derivatization of neomycin (due to the lack of chromophore groups in aminoglycosides). The method was successfully tested in the analysis of rubella vaccine containing trace residues of neomycin and in pharmaceutical compositions containing neomycin sulfate after solid phase extraction using an aminoglycoside imprinted polymer to improve selectivity in determinations.
Assuntos Scopus
Functionalized graphene , Imprinted polymers , Neomycin sulfates , Photoluminescence quenching , Rubella vaccines , Signal restoration , Solid-phase extraction , Three orders of magnitude , Aminoglycosides , Glutathione , Graphite , Iron , Limit of Detection , Luminescent Measurements , Microscopy, Electron, Scanning Transmission , Molecular Imprinting , Molecular Probes , Neomycin , Photoelectron Spectroscopy , Quantum Dots , Rubella Vaccine , Solid Phase Extraction , Spectrum Analysis, Raman , Temperature
DOI (Texto completo)