Efeito do omento no tratamento de superfície cartilaginosa submetida a osteoartrite experimental por Zymosan
Tipo
TCC
Data de publicação
2024-11-14
Periódico
Citações (Scopus)
Autores
Magalhães, Alice Bazan de
Daniel, Giovana Fistarol
Daniel, Giovana Fistarol
Orientador
Skare, Thelma Larocca
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
INTRODUÇÃO: A osteoartrite (OA) se caracteriza como um processo degenerativo, inflamatório e imunológico do sistema articular, levando à rigidez, dores e perda de movimentos na articulação afetada. Não existe, atualmente, um protocolo terapêutico definitivo para a OA. O omento, segundo a literatura científica tem demonstrado uma ampla variedade de funções terapêuticas, incluindo a participação na regeneração e neovascularização tecidual e na remodelação de estruturas lesionadas. OBJETIVO: Avaliar o efeito do omento na regeneração articular do joelho de ratos em modelo experimental de osteoartrite, induzido por Zymosan®. METODOLOGIA: Foram utilizados 40 ratos machos Wistar, distribuídos em 3 grupos: um grupo controle em que foi realizada gavagem com solução salina 0,9%, outro grupo tratado com combinação de sulfato de condroitina e glicosaminoglicano e o terceiro grupo tratado com aplicação intra-articular de omento na cartilagem lesada. Todos os grupos foram submetidos à indução de osteoartrite com Zymosan, à remoção de omento por laparotomia e artrotomia seguida de rafia. RESULTADOS: O experimento durou 60 dias. Dos 40 ratos, um morreu por causas naturais durante o experimento. Os resultados clínicos da osteoartrite, antes e depois das intervenções terapêuticas, mostraram que o grupo 3 (com enxerto de omento) obteve os menores escores médios, mas sem significância estatística. Os valores da circunferência dos joelhos foram maiores ao final do estudo, embora sem um padrão claro de aumento. O grupo tratado com omento apresentou escores semelhantes ao controle e ao tratado com condroitina e glicosamina em diversas avaliações, como incapacidade articular, análise histopatológica e análise radiológica, mostrando que tanto o omento como o tratamento padrão da doença não promoveram regeneração articular. CONCLUSÃO: Neste experimento, foi evidenciado que tanto o tratamento usual da OA, quanto a aplicação do fragmento do omento, não apresentaram capacidade de regeneração articular no joelho dos ratos.
Osteoarthritis (OA) is characterized as a degenerative, inflammatory, and immunologic process of the joint system, leading to stiffness, pain, and loss of movement in the affected joint. Currently, there is no definitive therapeutic protocol for OA. The omentum, according to scientific literature, has shown a wide range of therapeutic functions including in tissue regeneration, neovascularization, and the remodeling of injured structures. OBJECTIVE: To evaluate the effect of the omentum on joint regeneration in the knee of rats in an experimental model of osteoarthritis induced by Zymosan®. METHODS: A total of 40 male Wistar rats were used, distributed in 3 groups: a control group in which gavage was performed with 0.9% saline solution, another group treated with a combination of chondroitin sulfate and glycosaminoglycan and the third group treated with intra-articular application of omentum to the injured cartilage. All groups underwent osteoarthritis induction with Zymosan, omentum removal by laparotomy, and arthrotomy followed by raffia. RESULTS: The experiment lasted for 60 days. from 40 Wistar rats. Of the 40 rats, one died of natural causes during the experiment. The clinical results of osteoarthritis, before and after the therapeutic interventions, showed that group 3 (with omentum graft) obtained the lowest mean scores, but without statistical significance. Knee circumference values were higher at the end of the study, although without a clear pattern of increase. The group treated with omentum presented scores similar to the control group and the group treated with chondroitin and glucosamine in several evaluations, such as joint disability, histopathological analysis and radiological analysis, showing that both the omentum and the standard treatment of the disease did not promote joint regeneration. CONCLUSION: In this experiment, it was evidenced that both the usual treatment of OA and the application of the omentum fragment did not show joint regeneration capacity in the knee of the rats.
Osteoarthritis (OA) is characterized as a degenerative, inflammatory, and immunologic process of the joint system, leading to stiffness, pain, and loss of movement in the affected joint. Currently, there is no definitive therapeutic protocol for OA. The omentum, according to scientific literature, has shown a wide range of therapeutic functions including in tissue regeneration, neovascularization, and the remodeling of injured structures. OBJECTIVE: To evaluate the effect of the omentum on joint regeneration in the knee of rats in an experimental model of osteoarthritis induced by Zymosan®. METHODS: A total of 40 male Wistar rats were used, distributed in 3 groups: a control group in which gavage was performed with 0.9% saline solution, another group treated with a combination of chondroitin sulfate and glycosaminoglycan and the third group treated with intra-articular application of omentum to the injured cartilage. All groups underwent osteoarthritis induction with Zymosan, omentum removal by laparotomy, and arthrotomy followed by raffia. RESULTS: The experiment lasted for 60 days. from 40 Wistar rats. Of the 40 rats, one died of natural causes during the experiment. The clinical results of osteoarthritis, before and after the therapeutic interventions, showed that group 3 (with omentum graft) obtained the lowest mean scores, but without statistical significance. Knee circumference values were higher at the end of the study, although without a clear pattern of increase. The group treated with omentum presented scores similar to the control group and the group treated with chondroitin and glucosamine in several evaluations, such as joint disability, histopathological analysis and radiological analysis, showing that both the omentum and the standard treatment of the disease did not promote joint regeneration. CONCLUSION: In this experiment, it was evidenced that both the usual treatment of OA and the application of the omentum fragment did not show joint regeneration capacity in the knee of the rats.
Descrição
Palavras-chave
omento , osteoartrite , experimentação animal , cartilagem , omentum , osteoarthritis , animal experimentation , cartilage