An evaluation of 3-rhamnosylquercetin, a glycosylated form of quercetin, against the myotoxic and edematogenic effects of sPLA 2 from crotalus durissus terrificus

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dc.contributor.authorToyama D.D.O.
dc.contributor.authorGaeta H.H.
dc.contributor.authorPinho M.V.T.D.
dc.contributor.authorFerreira M.J.P.
dc.contributor.authorRomoff P.
dc.contributor.authorMatioli F.F.
dc.contributor.authorMagro A.J.
dc.contributor.authorFontes M.R.D.M.
dc.contributor.authorToyama M.H.
dc.date.accessioned2024-03-13T01:02:50Z
dc.date.available2024-03-13T01:02:50Z
dc.date.issued2014
dc.description.abstractThis paper shows the results of quercitrin effects on the structure and biological activity of secretory phospholipase (sPLA2) from Crotalus durissus terrificus, which is the main toxin involved in the pharmacological effects of this snake venom. According to our mass spectrometry and circular dichroism results, quercetin was able to promote a chemical modification of some amino acid residues and modify the secondary structure of C. d. terrificus sPLA2. Moreover, molecular docking studies showed that quercitrin can establish chemical interactions with some of the crucial amino acid residues involved in the enzymatic activity of the sPLA2, indicating that this flavonoid could also physically impair substrate molecule access to the catalytic site of the toxin. Additionally, in vitro and in vivo assays showed that the quercitrin strongly diminished the catalytic activity of the protein, altered its Vmax and Km values, and presented a more potent inhibition of essential pharmacological activities in the C. d. terrificus sPLA2, such as its myotoxicity and edematogenic effect, in comparison to quercetin. Thus, we concluded that the rhamnose group found in quercitrin is most likely essential to the antivenom activities of this flavonoid against C. d. terrificus sPLA2. © 2014 Daniela de Oliveira Toyama et al.
dc.description.volume2014
dc.identifier.doi10.1155/2014/341270
dc.identifier.issn2314-6141
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36513
dc.relation.ispartofBioMed Research International
dc.rightsAcesso Aberto
dc.titleAn evaluation of 3-rhamnosylquercetin, a glycosylated form of quercetin, against the myotoxic and edematogenic effects of sPLA 2 from crotalus durissus terrificus
dc.typeArtigo
local.scopus.citations16
local.scopus.eid2-s2.0-84896116210
local.scopus.subjectAnimals
local.scopus.subjectCircular Dichroism
local.scopus.subjectCrotalid Venoms
local.scopus.subjectCrotalus
local.scopus.subjectEdema
local.scopus.subjectEnzyme Assays
local.scopus.subjectGlycosylation
local.scopus.subjectMale
local.scopus.subjectMice
local.scopus.subjectMolecular Docking Simulation
local.scopus.subjectMuscle Cells
local.scopus.subjectPhospholipases A2, Secretory
local.scopus.subjectQuercetin
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84896116210&origin=inward
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