An evaluation of 3-rhamnosylquercetin, a glycosylated form of quercetin, against the myotoxic and edematogenic effects of sPLA 2 from crotalus durissus terrificus

Toyama D.D.O.; Gaeta H.H.; Pinho M.V.T.D.; Ferreira M.J.P.; Romoff P.; Matioli F.F.; Magro A.J.; Fontes M.R.D.M.; Toyama M.H.

An evaluation of 3-rhamnosylquercetin, a glycosylated form of quercetin, against the myotoxic and edematogenic effects of sPLA 2 from crotalus durissus terrificus

Tipo

Artigo

Data de publicação

2014

Periódico

BioMed Research International

Citações (Scopus)

16

Autores

Toyama D.D.O.
Gaeta H.H.
Pinho M.V.T.D.
Ferreira M.J.P.
Romoff P.
Matioli F.F.
Magro A.J.
Fontes M.R.D.M.
Toyama M.H.

Resumo

This paper shows the results of quercitrin effects on the structure and biological activity of secretory phospholipase (sPLA2) from Crotalus durissus terrificus, which is the main toxin involved in the pharmacological effects of this snake venom. According to our mass spectrometry and circular dichroism results, quercetin was able to promote a chemical modification of some amino acid residues and modify the secondary structure of C. d. terrificus sPLA2. Moreover, molecular docking studies showed that quercitrin can establish chemical interactions with some of the crucial amino acid residues involved in the enzymatic activity of the sPLA2, indicating that this flavonoid could also physically impair substrate molecule access to the catalytic site of the toxin. Additionally, in vitro and in vivo assays showed that the quercitrin strongly diminished the catalytic activity of the protein, altered its Vmax and Km values, and presented a more potent inhibition of essential pharmacological activities in the C. d. terrificus sPLA2, such as its myotoxicity and edematogenic effect, in comparison to quercetin. Thus, we concluded that the rhamnose group found in quercitrin is most likely essential to the antivenom activities of this flavonoid against C. d. terrificus sPLA2. © 2014 Daniela de Oliveira Toyama et al.

Assuntos Scopus

Animals , Circular Dichroism , Crotalid Venoms , Crotalus , Edema , Enzyme Assays , Glycosylation , Male , Mice , Molecular Docking Simulation , Muscle Cells , Phospholipases A2, Secretory , Quercetin