The Effect of Stool Transplantation on Weight Control in Obese Rats
Tipo
Artigo
Data de publicação
2021
Periódico
Journal of Coloproctology
Citações (Scopus)
0
Autores
Klas C.F.
Reboucas R.L.
Diosti G.D.M.
Osman K.
Carmona L.F.P.
Kubrusly L.F.
Marques C.M.
Reboucas R.L.
Diosti G.D.M.
Osman K.
Carmona L.F.P.
Kubrusly L.F.
Marques C.M.
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Título de Volume
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Resumo
© 2021 Elsevier Editora Ltda. All rights reserved.Introduction: Obesity is defined as a multifactorial metabolic syndrome in which there is an excessive number of fat cells within the tissues. It is discussed that intestinal microbiota might have a relevant relation with obesity, since it is relevantly altered in obese patients. Objectives: To assess the effect of stool transplantation (ST) in the condition of obesity and its outcomes in an experimental model of cafeteria diet by analyzing histology and weight gain. Methods: Forty male Wistar rats were randomly assigned to 5 groups: control (CO), control with antibiotics (CO + ATB), obesity (CAF + ATB), stool transplantation (ATB + ST) and obesity with stool transplantation (CAF + ATB + ST). During the experiment, obesity induction groups received cafeteria diet, whereas the remaining groups had normal diet ad libitum. After 3 months, daily ST was carried out for 8 weeks by gavage procedure. The animals were euthanized, and the small intestine was harvested for further analysis. Results: It was observed that before starting the ST, the cafeteria and normal diet groups had significant weight difference (p < 0,0001). In the comparison between CAF + ATB and CAF + ATB + ST during the gavage period, the CAF + ATB + ST group presented lower weight gain (p = 0.0017). The histopathological evaluation show that the ATB + ST group did not present intestinal crypt distortion. Conclusion: Cafeteria diet resulted in an expected weight gain. In relation to the ST, it has been shown that the procedure is effective in reducing weekly weight gain. Apparently, there was no induction of disabsortive syndrome in nonobese animals that received ST.