Ratos wistar de biotério x ratos twister de estimação: um estudo de comparação comportamental e possíveis influências para estudos científicos
Tipo
TCC
Data de publicação
2023-06
Periódico
Citações (Scopus)
Autores
Aprigliano, Júlia Vicentini
Orientador
Camargo, Esther Lopes Ricci Adari
Título da Revista
ISSN da Revista
Título de Volume
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Resumo
Ratos são os animais mais utilizados em trabalhos científicos pela sua semelhança fisiológica e genética comparada aos seres humanos, além de serem mamíferos de porte pequeno com alta reprodutibilidade, conseguem ocupar pouco espaço, facilitando sua manipulação e manutenção. Por isso, é importante entender quais são seus comportamentos naturais para que os resultados das pesquisas científicas tenham maior êxito. Assim, o presente estudo teve como objetivo estudar diferenças comportamentais de Rattus norvegicus, entre ratos wistar utilizados em biotérios, já que, no geral, vivem em condição de alojamento com pouco estímulo social, sem enriquecimento ambiental e com delimitação de espaço, com ratos twister de estimação, que têm alojamento espaçoso e com enriquecimento ambiental, a fim de compreender a possível influência dos diferentes tipos de criação nos resultados de pesquisas científicas. Para a realização dos experimentos, foram utilizados 12 ratos adultos, divididos em dois grupos: ratos que residem nas instalações de biotérios, seguindo o padrão de moradia deles, e ratos de estimação que residem na casa da aluna pesquisadora, em alojamentos enriquecidos. A avaliação comportamental foi realizada por testes comportamentais, incluindo campo aberto, caixa claro/escuro e grooming espontâneo. Os resultados do estudo demonstraram que: (1) os animais de biotério apresentaram menor frequência de locomoção e levantar e menor frequência no centro e periferia do aparato no teste do campo aberto; (2) os animais de biotério cruzaram menos e tiveram menor permanência nos compartimentos do aparato no teste da caixa claro/escuro; (3) os animais de estimação realizaram grooming por mais tempo nos 3 testes realizados. Os dados em conjunto sugerem que os ratos de estimação se sentiram mais confortáveis para explorar os aparatos e demonstraram menos neofobia, possivelmente em decorrência da sua criação. Assim, é possível concluir que o tipo de criação e formas diversas de enriquecimentos ambientais, sociais e alimentares podem interferir na emocionalidade dos animais utilizados como modelo de experimentação.
Rats are the most used animals in scientific work due to their physiological and genetic similarity to humans. Therefore, it is important to understand what their natural behaviors are so that the results of scientific research are more successful. Therefore, the aim of this study is compare behavioral differences of Rattus norvegicus, between wistar rats, used in biotheriums, since, in general, they live in housing conditions with little social stimulation, without environmental enrichment and with space delimitation, with pet rats (twister rat), which have more housing space and with environmental enrichment, in order to understand the influence and interference of different types of breeding in the results of scientific research. For the experiments, 12 adult rats were used, divided into two groups: rats that reside in biotherium facilities, following their housing pattern, and pet rats that reside in the researcher student's house, in enriched accommodation. Behavioral assessment was performed by behavioral tests, including open field, light/dark box and spontaneous grooming. The results of the study demonstrated that: (1) biotherium animals showed lower frequency of locomotion and lifting and lower frequency in the center and periphery of the apparatus in the open field test; (2) biotheriumanimals crossed less and spent less time in the apparatus compartments in the light/dark box test; (3) pet rats groomed longer in the 3 tests performed. The graphics data suggest that pet rats felt more comfortable exploring the apparatus and showed less neophobia, possibly as a result of their breeding. Thus, it is possible to conclude that the type of breeding and different forms of environmental, social and food enrichment can interfere with the emotionality of the animals used as an experimental model.
Rats are the most used animals in scientific work due to their physiological and genetic similarity to humans. Therefore, it is important to understand what their natural behaviors are so that the results of scientific research are more successful. Therefore, the aim of this study is compare behavioral differences of Rattus norvegicus, between wistar rats, used in biotheriums, since, in general, they live in housing conditions with little social stimulation, without environmental enrichment and with space delimitation, with pet rats (twister rat), which have more housing space and with environmental enrichment, in order to understand the influence and interference of different types of breeding in the results of scientific research. For the experiments, 12 adult rats were used, divided into two groups: rats that reside in biotherium facilities, following their housing pattern, and pet rats that reside in the researcher student's house, in enriched accommodation. Behavioral assessment was performed by behavioral tests, including open field, light/dark box and spontaneous grooming. The results of the study demonstrated that: (1) biotherium animals showed lower frequency of locomotion and lifting and lower frequency in the center and periphery of the apparatus in the open field test; (2) biotheriumanimals crossed less and spent less time in the apparatus compartments in the light/dark box test; (3) pet rats groomed longer in the 3 tests performed. The graphics data suggest that pet rats felt more comfortable exploring the apparatus and showed less neophobia, possibly as a result of their breeding. Thus, it is possible to conclude that the type of breeding and different forms of environmental, social and food enrichment can interfere with the emotionality of the animals used as an experimental model.
Descrição
Palavras-chave
comportamneto animal , ratos wistar , ratos twister , enriquecimento ambiental , animal behavior , wistar rats , twister Rats , environmental enrichment