Impact of Long-term Storage in 4% Formaldehyde on Immunophenotypic Markers in Glutaraldehyde-treated Bovine Pericardium

Página do item simplificado
dc.contributor.authorGewehr D.M.
dc.contributor.authorCarvalho P.E.P.
dc.contributor.authorIzar F.P.
dc.contributor.authorHaddad A.G.
dc.contributor.authorFalkenbach Tenius V.D.
dc.contributor.authorGiovanini A.F.
dc.contributor.authorKubrusly L.F.
dc.date.accessioned2024-09-01T06:15:48Z
dc.date.available2024-09-01T06:15:48Z
dc.date.issued2024
dc.description.abstract© 2024 Lippincott Williams and Wilkins. All rights reserved.Bovine pericardium (BP) is widely used as a biomaterial for tissue engineering. Glutaraldehyde and formaldehyde are commonly employed in the reticulation processes to enhance the material's resistance and preservation. In this study, we assessed the impact of long-term storage in 4% formaldehyde on the quantitative expression of immunophenotypic markers in glutaraldehyde-treated BP. Histologic and immunohistochemical analyses were performed on 2 BP patches, manufactured in 2009 and 2020, respectively. Braile Biomédica provided the BP patches. Sections of BP were stained with H&E, Weigert, and picrosirius red, followed by immunolabeling for vimentin, laminin 5, collagen I, and collagen IV using a standardized protocol. Microscopic images were captured using light and polarized microscopy, and the area of the antibody signal was quantified using Image J Software. Histologic analysis showed no autolysis or significant changes in the patches. Immunohistochemical analysis revealed a diffuse distribution of collagen I and collagen IV throughout the connective tissue of the patches. The 2020 specimen exhibited higher expression levels of collagen I (21.36%) and collagen IV (24.67%) compared with the 2009 specimen (collagen I: 15.87%; collagen IV: 12.02%). Laminin did not show reactivity in either specimen. Notably, vimentin immunopositivity differed significantly between the patches, with a larger area of expression observed in the 2020 patch (54%) compared with the 2009 patch (13%). In summary, there were no substantial differences in immunophenotypic expression between the 2009 and 2020 BP patches, except for the higher vimentin expression in the 2020 BP patch.
dc.identifier.doi10.1097/PAI.0000000000001221
dc.identifier.issnNone
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/39259
dc.relation.ispartofApplied Immunohistochemistry and Molecular Morphology
dc.rightsAcesso Restrito
dc.subject.otherlanguagebioprosthesis
dc.subject.otherlanguageglutaraldehyde-stabilized graft
dc.subject.otherlanguagepericardium-formaldehyde heart valve prosthesis
dc.titleImpact of Long-term Storage in 4% Formaldehyde on Immunophenotypic Markers in Glutaraldehyde-treated Bovine Pericardium
dc.typeArtigo
local.scopus.citations0
local.scopus.eid2-s2.0-85202192455
local.scopus.updated2025-04-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85202192455&origin=inward
Arquivos
Coleções
Artigos de periódico