Characterization of pomegranate peel extracts obtained using different solvents and their effects on cell cycle and apoptosis in leukemia cells

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dc.contributor.authorTamborlin L.
dc.contributor.authorSumere B.R.
dc.contributor.authorde Souza M.C.
dc.contributor.authorPestana N.F.
dc.contributor.authorAguiar A.C.
dc.contributor.authorEberlin M.N.
dc.contributor.authorSimabuco F.M.
dc.contributor.authorRostagno M.A.
dc.contributor.authorLuchessi A.D.
dc.date.accessioned2024-03-12T23:46:40Z
dc.date.available2024-03-12T23:46:40Z
dc.date.issued2020
dc.description.abstract© 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.Pomegranate (Punica granatum L.) has been used in traditional herbal medicine by several cultures as an anti-inflammatory, antioxidant, antihyperglycemic, and for treatment and prevention of cancer and other diseases. Different parts of the fruit, extraction methods, and solvents can define the chemical profile of the obtained extracts and their biological activities. This study aimed to characterize the chemical profile of peel extracts collected using different extraction solvents and their biological effects on the cell cycle and apoptosis of THP-1 leukemic cells. Aqueous extract presented the highest content of punicalagins (α pun = 562.26 ± 47.14 mg/L and β pun = 1,251.13 ± 22.21 mg/L) and the lowest content of ellagic acid (66.38 ± 0.21 mg/L), and it promoted a significant impairment of the cell cycle S phase. In fact, punicalagin-enriched fraction, but not an ellagic acid-enriched fraction, caused an S phase cell cycle arrest. All extracts increased the number of apoptotic cells. Punicalagin-enriched fraction increased the percentage of cells with fragmented DNA, which was intensified by ellagic acid combination. The treatment combining punicalagin and ellagic acid fractions increased the apoptotic cleaved PARP1 protein and reduced the activation of the growth-related mTOR pathway. Thus, these results evidence that solvent choice is critical for the phenolic compounds profile of pomegranate peel extracts and their biological activities.
dc.description.firstpage5483
dc.description.issuenumber10
dc.description.lastpage5496
dc.description.volume8
dc.identifier.doi10.1002/fsn3.1831
dc.identifier.issn2048-7177
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/34908
dc.relation.ispartofFood Science and Nutrition
dc.rightsAcesso Aberto
dc.subject.otherlanguageapoptosis
dc.subject.otherlanguagebiological activity
dc.subject.otherlanguagecell cycle
dc.subject.otherlanguageleukemia
dc.subject.otherlanguagepomegranate peel extracts
dc.subject.otherlanguagepunicalagin
dc.titleCharacterization of pomegranate peel extracts obtained using different solvents and their effects on cell cycle and apoptosis in leukemia cells
dc.typeArtigo
local.scopus.citations21
local.scopus.eid2-s2.0-85089984195
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85089984195&origin=inward
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