Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse

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dc.contributor.authorPreite N.Z.
dc.contributor.authorDo Nascimento B.P.P.
dc.contributor.authorMuller C.R.
dc.contributor.authorAmerico A.L.V.
dc.contributor.authorHiga T.S.
dc.contributor.authorEvangelista F.S.
dc.contributor.authorLancellotti C.L.
dc.contributor.authorHenriques F.S.
dc.contributor.authorBatista M.L.
dc.contributor.authorBianco A.C.
dc.contributor.authorRibeiro M.O.
dc.date.accessioned2024-03-13T00:54:59Z
dc.date.available2024-03-13T00:54:59Z
dc.date.issued2016
dc.description.abstract© 2016 Society for Endocrinology.The brown adipose tissue (BAT) mediates adaptive changes in metabolic rate by responding to the sympathetic nervous system through β-adrenergic receptors (AR). Here, we wished to define the role played by the ARβ3 isoform in this process. This study focused on the ARβ3 knockout mice (ARβ3KO), including responsiveness to cold exposure, diet-induced obesity, intolerance to glucose, dyslipidaemia and lipolysis in white adipose tissue (WAT). ARβ3KO mice defend core temperature during cold exposure (4°C for 5 h), with faster BAT thermal response to norepinephrine (NE) infusion when compared with wild-type (WT) mice. Despite normal BAT thermogenesis, ARβ3KO mice kept on a high-fat diet (HFD; 40% fat) for 8 weeks exhibited greater susceptibility to diet-induced obesity, markedly increased epididymal adipocyte area with clear signs of inflammation. The HFD-induced glucose intolerance was similar in both groups but serum hypertriglyceridemia and hypercholesterolemia were less intense in ARβ3KO animals when compared with WT controls. Isoproterenol-induced lipolysis in isolated white adipocytes as assessed by glycerol release was significantly impaired in ARβ3KO animals despite normal expression of key proteins involved in lipid metabolism. In conclusion, ARβ3 inactivation does not affect BAT thermogenesis but increases susceptibility to dietinduced obesity by dampening WAT lipolytic response to adrenergic stimulation.
dc.description.firstpage259
dc.description.issuenumber3
dc.description.lastpage269
dc.description.volume231
dc.identifier.doi10.1530/JOE-16-0199
dc.identifier.issn1479-6805
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36073
dc.relation.ispartofJournal of Endocrinology
dc.rightsAcesso Aberto
dc.subject.otherlanguageadaptive thermogenesis
dc.subject.otherlanguagebrown adipose tissue
dc.subject.otherlanguagelipolysis
dc.subject.otherlanguageObesity
dc.subject.otherlanguageβ3 adrenergic receptor
dc.titleDisruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse
dc.typeArtigo
local.scopus.citations24
local.scopus.eid2-s2.0-85004178518
local.scopus.subjectAdipose Tissue, Brown
local.scopus.subjectAdipose Tissue, White
local.scopus.subjectAdiposity
local.scopus.subjectAnimals
local.scopus.subjectCold Temperature
local.scopus.subjectDiet, High-Fat
local.scopus.subjectLipid Metabolism
local.scopus.subjectLipolysis
local.scopus.subjectMale
local.scopus.subjectMice
local.scopus.subjectMice, Knockout
local.scopus.subjectNorepinephrine
local.scopus.subjectObesity
local.scopus.subjectReceptors, Adrenergic, beta-3
local.scopus.subjectThermogenesis
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85004178518&origin=inward
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