Flow-injection system with enzyme reactor for differential amperometric determination of hydrogen peroxide in rainwater

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dc.contributor.authorMatos R.C.
dc.contributor.authorPedrotti J.J.
dc.contributor.authorAngnes L.
dc.date.accessioned2024-03-13T01:46:53Z
dc.date.available2024-03-13T01:46:53Z
dc.date.issued2001
dc.description.abstractDifferential determinations of hydrogen peroxide (H2O2) have been performed by amperometry, combining flow-injection analysis (FIA) and a tubular reactor containing immobilized enzymes. A gold microelectrode modified by electrochemical deposition of platinum was employed as working electrode. Hydrogen peroxide was quantified in rainwater using amperometric differential measurements at +0.60 V versus Ag/AgCl(sat). For the enzymatic consumption of H2O2, a tubular reactor containing immobilized catalase was constructed, using a novel way for immobilization of enzymes on Amberlite IRA-743 resin. The linear dynamic range in H2O2 extends from 1 to 100 × 10-6 mol/l, at pH 7.0. At flow rate of 2.0 ml/min and injecting 150 μl sample volumes, the sampling frequency of the 90 determinations/h is afforded. The reproducibility of the current peaks for hydrogen peroxide in 10-5 mol/l range concentration shown a R.S.D. better than 1%. The detection limit of the method is 2.9 × 10-7 mol/l (1.5 ng of H2O2 in a 150 μl sample). The rainwater samples analyses were compared with the parallel amperometric determinations using static mercury electrode and by spectrophotometry, showing very good correlation between the three methods. © 2001 Elsevier Science B.V. All rights reserved.
dc.description.firstpage73
dc.description.issuenumber1
dc.description.lastpage79
dc.description.volume441
dc.identifier.doi10.1016/S0003-2670(01)01094-7
dc.identifier.issn0003-2670
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/38033
dc.relation.ispartofAnalytica Chimica Acta
dc.rightsAcesso Restrito
dc.subject.otherlanguageCatalase
dc.subject.otherlanguageHydrogen peroxide
dc.subject.otherlanguageModified microelectrode
dc.subject.otherlanguagePlatinum
dc.subject.otherlanguageRainwater
dc.titleFlow-injection system with enzyme reactor for differential amperometric determination of hydrogen peroxide in rainwater
dc.typeArtigo
local.scopus.citations51
local.scopus.eid2-s2.0-0035899514
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0035899514&origin=inward
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