A betabaculovirus encoding a gp64 homolog

Ardisson-Araujo D.M.P.; Pereira B.T.; Melo F.L.; Ribeiro B.M.; Bao S.N.; Zanotto P.M.A.; Moscardi F.; Kitajima E.W.; Sosa-Gomez D.R.; Wolff J.L.C.

A betabaculovirus encoding a gp64 homolog

Tipo

Artigo

Data de publicação

2016

Periódico

BMC Genomics

Citações (Scopus)

10

Autores

Ardisson-Araujo D.M.P.
Pereira B.T.
Melo F.L.
Ribeiro B.M.
Bao S.N.
Zanotto P.M.A.
Moscardi F.
Kitajima E.W.
Sosa-Gomez D.R.
Wolff J.L.C.

Resumo

© 2016 Ardisson-Araújo et al.Background: A betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. Results: The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister group to a cluster formed by ChocGV, ErelGV, PiraGV isolates, ClanGV, CaLGV, CpGV, CrleGV, AdorGV, PhopGV and EpapGV. Surprisingly, we found in the DisaGV genome a G protein-coupled receptor related to lepidopteran and other insect virus genes and a gp64 homolog, which is likely a product of horizontal gene transfer from Group 1 alphabaculoviruses. Conclusion: DisaGV represents a distinct lineage of the genus Betabaculovirus. It is closely related to the CpGV-related group and presents the smallest genome in size so far. Remarkably, we found a homolog of gp64, which was reported solely in group 1 alphabaculovirus genomes so far.

Assuntos Scopus

Baculoviridae , Base Composition , Base Sequence , Brazil , Gene Order , Genome, Viral , Genomics , Molecular Sequence Data , Open Reading Frames , Phylogeny , Saccharum , Viral Envelope Proteins , Viral Proteins