Urinary amino acid metabolomic profiling and its association with childhood obesity in prepubescent individuals

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dc.contributor.authorPassadore M.D.
dc.contributor.authorAzinheira Nobrega Cruz N.
dc.contributor.authorBocato M.Z.
dc.contributor.authorFerreira L.D.A.
dc.contributor.authorIcimoto M.Y.
dc.contributor.authorMolina M.D.C.B.
dc.contributor.authorMill J.G.
dc.contributor.authorBarbosa Junior F.
dc.contributor.authorCasarini D.E.
dc.contributor.authorOliveira L.C.G.D.
dc.date.accessioned2025-06-01T06:14:08Z
dc.date.available2025-06-01T06:14:08Z
dc.date.issued2025
dc.description.abstractCopyright © 2025 Passadore, Azinheira Nobrega Cruz, Bocato, Ferreira, Icimoto, Molina, Mill, Barbosa Junior, Casarini and Oliveira.Introduction: Amino acids are fundamental in several metabolic processes, and their levels can reflect metabolism impairments that contribute to obesity and related diseases. Our objective was to identify a urinary amino acid fingerprint in obese and overweight children in prepuberty and to correlate this profile with cardiometabolic alterations. Methods: The study included 110 children, boys and girls aged 9–10 years, they were classified according to their BMI-for-age (Body Mass Index for age) into three groups: normal weight (NW) (n = 45), overweight (OW) (n = 21), and obese (OB) (n = 44). The 12-h urine samples were analyzed by LC-MS/MS to quantify 47 amino acids using the Amino Acids Analysis Kit (Zivak®, Turkey), values were corrected by creatinine concentration. Anthropometric measurements, cardiovascular parameters, and biochemical profiles were assessed following standard protocols. Results: When compared to NW, anthropometric measures, systolic and diastolic blood pressure, and serum uric acid levels were progressively elevated in the OW and OB groups. The OB group was characterized by elevated alpha-aminoadipic acid, asparagine, cystathionine, 1-methyl-histidine, serine, tryptophan, phenylalanine, and tyrosine. In contrast, the OW group presented the most expressive levels of glutamine, alpha-diaminopimelic, and sarcosine. Discussion: Our findings indicate that obese and overweight children exhibit a particular urinary amino acid fingerprint which is similar to that reported in studies with plasma. The altered amino acids, particularly tyrosine, are frequently associated with impairments in glucose homeostasis, insulin resistance, and diabetes mellitus type 2. Potential mechanisms for increasing the levels of these amino acids in excess of weight may include enhanced protein degradation and impaired oxidative metabolism.
dc.description.volume16
dc.identifier.doi10.3389/fphys.2025.1524939
dc.identifier.issnNone
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/40905
dc.relation.ispartofFrontiers in Physiology
dc.rightsAcesso Restrito
dc.subject.otherlanguagebiomarkers
dc.subject.otherlanguagechildhood obesity
dc.subject.otherlanguagemetabolomics
dc.subject.otherlanguageprepuberty
dc.subject.otherlanguageurinary amino acids
dc.titleUrinary amino acid metabolomic profiling and its association with childhood obesity in prepubescent individuals
dc.typeArtigo
local.scopus.citations0
local.scopus.eid2-s2.0-105004886382
local.scopus.updated2025-06-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105004886382&origin=inward
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