Mass spectrometry imaging as a potential technique for diagnostic of Huanglongbing disease using fast and simple sample preparation

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dc.contributor.authorde Moraes Pontes J.G.
dc.contributor.authorVendramini P.H.
dc.contributor.authorFernandes L.S.
dc.contributor.authorde Souza F.H.
dc.contributor.authorPilau E.J.
dc.contributor.authorEberlin M.N.
dc.contributor.authorMagnani R.F.
dc.contributor.authorWulff N.A.
dc.contributor.authorFill T.P.
dc.date.accessioned2024-03-12T23:45:55Z
dc.date.available2024-03-12T23:45:55Z
dc.date.issued2020
dc.description.abstract© 2020, The Author(s).Huanglongbing (HLB) is a disease of worldwide incidence that affects orange trees, among other commercial varieties, implicating in great losses to the citrus industry. The disease is transmitted through Diaphorina citri vector, which inoculates Candidatus Liberibacter spp. in the plant sap. HLB disease lead to blotchy mottle and fruit deformation, among other characteristic symptoms, which induce fruit drop and affect negatively the juice quality. Nowadays, the disease is controlled by eradication of sick, symptomatic plants, coupled with psyllid control. Polymerase chain reaction (PCR) is the technique most used to diagnose the disease; however, this methodology involves high cost and extensive sample preparation. Mass spectrometry imaging (MSI) technique is a fast and easily handled sample analysis that, in the case of Huanglongbing allows the detection of increased concentration of metabolites associated to the disease, including quinic acid, phenylalanine, nobiletin and sucrose. The metabolites abieta-8,11,13-trien-18-oic acid, suggested by global natural product social molecular networking (GNPS) analysis, and 4-acetyl-1-methylcyclohexene showed a higher distribution in symptomatic leaves and have been directly associated to HLB disease. Desorption electrospray ionization coupled to mass spectrometry imaging (DESI-MSI) allows the rapid and efficient detection of biomarkers in sweet oranges infected with Candidatus Liberibacter asiaticus and can be developed into a real-time, fast-diagnostic technique.
dc.description.issuenumber1
dc.description.volume10
dc.identifier.doi10.1038/s41598-020-70385-4
dc.identifier.issn2045-2322
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/34865
dc.relation.ispartofScientific Reports
dc.rightsAcesso Aberto
dc.titleMass spectrometry imaging as a potential technique for diagnostic of Huanglongbing disease using fast and simple sample preparation
dc.typeArtigo
local.scopus.citations29
local.scopus.eid2-s2.0-85089262747
local.scopus.subjectAnimals
local.scopus.subjectCitrus
local.scopus.subjectCyclohexanes
local.scopus.subjectDiagnosis
local.scopus.subjectDisease Vectors
local.scopus.subjectDNA, Bacterial
local.scopus.subjectHemiptera
local.scopus.subjectMass Spectrometry
local.scopus.subjectPlant Diseases
local.scopus.subjectPlant Leaves
local.scopus.subjectPolymerase Chain Reaction
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85089262747&origin=inward
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