Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts
Tipo
Artigo
Data de publicação
2014
Periódico
Quimica Nova
Citações (Scopus)
34
Autores
De Oliveira S.
De Souza G.A.
Eckert C.R.
Silva T.A.
Sobral E.S.
Favero O.A.
Ferreira M.J.P.
Romoff P.
Baader W.J.
De Souza G.A.
Eckert C.R.
Silva T.A.
Sobral E.S.
Favero O.A.
Ferreira M.J.P.
Romoff P.
Baader W.J.
Orientador
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter.