Análise em amostras glandulares cervico-vaginais da presença de HPV
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Tipo
TCC
Data de publicação
2018-12
Periódico
Citações (Scopus)
Autores
Bitun, Marina Anchieta
Orientador
Pincinato, Éder de Carvalho
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
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Resumo
O Papilomavírus humano é o virus que causa a doença sexualmente
transmissível mais comum e frequente da atualidade, levando a preocupação
mundial, pelo fato de sua correlação com o câncer. Após duas décadas de
descoberta, estimativas recentes indicam que infecções pelo HPV, são
responsáveis por aproximadamente 500.000 casos de câncer e associados a
275.000 casos de morte, anualmente, justificando a realização deste trabalho,
que teve por objetivo detectar e genotipar o HPV de alto e baixo risco oncológico
em amostras da cérvice uterina de pacientes com diagnostico citológico de
células glandulares atípicas. Para atingirmos estes objetivos, as amostras
biológicas foram coletadas em meio líquido, seguida de extração de DNA por kit
comercial (Promega) e a detecção e genotipagem do HPV foi realizada por
Nested Multiplex PCR (NMPCR). De acordo com os resultados obtidos, todas as
amostras analisadas apresentaram pelo menos 1 tipo de HPV. O tipo de HPV
mais frequente foi o 18 (100%) e menos frequente foi o 33 (28,6%). Portanto,
conclui-se que houve uma alta prevalência do HPV, principalmente os de alto
risco oncogênico, o que contribui para um prognóstico desfavorável das
pacientes estudadas. Estes resultados devem ser futuramente confirmados e
mais pacientes devem ser incluídos, a fim de confirmar os dados apresentados
neste trabalho.
The human papillomavirus is the most common and frequent sexually transmitted disease today, leading to worldwide concern, because of its correlation with cancer. After two decades of discovery, recent estimates indicate that HPV infections are responsible for approximately 500,000 cases of cancer and associated with 275,000 cases of death annually, justifying the accomplishment of this work, which aimed to detect and genotype the HPV of high and low oncological risk in samples of the uterine cervix of patients with cytological diagnosis of atypical glandular cells. To achieve these objectives, the biological samples were collected in liquid medium, followed by DNA extraction by commercial kit (Promega) and HPV detection and genotyping was performed by Nested Multiplex PCR (NMPCR). According to the results obtained, all samples analyzed showed at least 1type of HPV. The most frequent type of HPV was 18 (100%) and less frequent was 33 (28.6%). Therefore, it was concluded that there was a high prevalence of HPV, especially those with high oncogenic risk, which contributes to an unfavorable prognosis of the patients studied. These results should be confirmed in the future and more patients should be included in order to confirm the data presented in this study.
The human papillomavirus is the most common and frequent sexually transmitted disease today, leading to worldwide concern, because of its correlation with cancer. After two decades of discovery, recent estimates indicate that HPV infections are responsible for approximately 500,000 cases of cancer and associated with 275,000 cases of death annually, justifying the accomplishment of this work, which aimed to detect and genotype the HPV of high and low oncological risk in samples of the uterine cervix of patients with cytological diagnosis of atypical glandular cells. To achieve these objectives, the biological samples were collected in liquid medium, followed by DNA extraction by commercial kit (Promega) and HPV detection and genotyping was performed by Nested Multiplex PCR (NMPCR). According to the results obtained, all samples analyzed showed at least 1type of HPV. The most frequent type of HPV was 18 (100%) and less frequent was 33 (28.6%). Therefore, it was concluded that there was a high prevalence of HPV, especially those with high oncogenic risk, which contributes to an unfavorable prognosis of the patients studied. These results should be confirmed in the future and more patients should be included in order to confirm the data presented in this study.
Descrição
Palavras-chave
papilomavírus humano (HPV) , nested multiplex PCR , câncer do colo do útero , alto e baixo risco oncológico , human papillomavirus (HPV) , nested multiplex PCR , cancer of the cervix , high and low cancer risk