Development and characterization of alginate-derived bioadhesive films incorporated with anti-infective lectins for application in the treatment of oral candidiasis

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dc.contributor.authorBazan J.M.N.
dc.contributor.authorChagas V.L.
dc.contributor.authorSilva R.G.
dc.contributor.authorSoeiro Silva I.S.
dc.contributor.authorNantes Araujo J.G.
dc.contributor.authorSilva L.D.S.
dc.contributor.authorBatista K.L.R.
dc.contributor.authorSilva R.R.D.S.
dc.contributor.authorCorreia M.T.D.S.
dc.contributor.authorSousa J.C.D.S.
dc.contributor.authorMonteiro C.D.A.
dc.contributor.authorTofanello A.
dc.contributor.authorGarcia W.
dc.contributor.authorCarvalho E.M.
dc.contributor.authorTeixeira C.S.
dc.contributor.authorNascimento da Silva L.C.
dc.date.accessioned2024-03-12T19:07:50Z
dc.date.available2024-03-12T19:07:50Z
dc.date.issued2023
dc.description.abstract© 2023This study reports the characterization of alginate-derived bioadhesive films containing the lectins ConBr (from Canavalia brasiliensis Mart.) and MaL (from Machaerium acutifolium Vogel.). The anti-infective properties were evaluated using Tenebrio molitor larvae infected with an oral isolate of Candida albicans (CA40) (1 × 104 yeast/animal). Untreated larvae showed a median survival of 1.5 days and a survival rate of 40%. Both lectins (128 μg/kg/larvae) showed prophylactic effects (survival rates: 80%–90%); however, only MaL significantly increased larval survival (70%) in the treatment assays. Bioadhesive films were developed using sodium alginate, and each lectin was incorporated at 50 μg/mL of filmogenic solution. All films (pH 6.4) showed similar micromorphology with a slightly granular appearance. The lectins-loaded films exhibited more irregular surfaces than the control. The proteins were released in similar patterns. FTIR analysis confirmed the modification of sodium alginate due to the chemical interaction with lectins. All films exhibited a two-step weight loss pattern in TGA analysis. In the ex vivo mucoadhesive assay using bovine jugal mucosa, the highest value of maximum detachment force was found for ConBr-loaded films (0.56 ± 0.04 N) (p < 0.01). The other formulations showed similar adhesiveness (0.48 and 0.47 N). In general, the lectins did not induce significant changes in the physicochemical and mechanical characteristics of the alginate films. The data obtained in this work show, for the first time, the in vivo anti-infective effects of ConBr and MaL against C. albicans. The lectins-containing alginate films presented parameters compatible with their application in the oral cavity.
dc.description.volume90
dc.identifier.doi10.1016/j.jddst.2023.105114
dc.identifier.issn1773-2247
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/33985
dc.relation.ispartofJournal of Drug Delivery Science and Technology
dc.rightsAcesso Restrito
dc.subject.otherlanguageDrug delivery
dc.subject.otherlanguageOral candidiasis
dc.subject.otherlanguagePolysaccharide
dc.titleDevelopment and characterization of alginate-derived bioadhesive films incorporated with anti-infective lectins for application in the treatment of oral candidiasis
dc.typeArtigo
local.scopus.citations4
local.scopus.eid2-s2.0-85175440564
local.scopus.updated2025-04-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85175440564&origin=inward
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