Chronic treatment with d-chiro-inositol prevents autonomic and somatic neuropathy in STZ-induced diabetic mice

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dc.contributor.authorFarias V.X.
dc.contributor.authorMacedo F.H.P.
dc.contributor.authorOquendo M.B.
dc.contributor.authorTome A.R.
dc.contributor.authorBao S.N.
dc.contributor.authorCintra D.O.S.
dc.contributor.authorSantos C.F.
dc.contributor.authorAlbuquerque A.A.C.
dc.contributor.authorHeimark D.B.
dc.contributor.authorLarner J.
dc.contributor.authorFonteles M.C.
dc.contributor.authorLeal-Cardoso J.H.
dc.contributor.authorNascimento N.R.F.
dc.date.accessioned2024-03-13T01:29:37Z
dc.date.available2024-03-13T01:29:37Z
dc.date.issued2011
dc.description.abstractAim: d-chiro-inositol (DCI) has been shown to prevent and reverse endothelial dysfunction in diabetic rats and rabbits. The present study evaluates the preventive effect of DCI on experimental diabetic neuropathy (DN). Methods: Streptozotocin-induced (STZ) diabetic mice were treated by oral gavage for 60 days with DCI (20 mg/kg/12 h) or saline (NaCl 0.9%; 0.1 ml/10 g/12 h; Diab) and compared with euglycaemic groups treated with saline (0.1 ml/10 g/12 h; Eugly). We compared the response of the isolated sciatic nerve, corpora cavernosa or vas deferens to electrical stimulation. Results: The electrically evoked compound action potential of the sciatic nerve was greatly blunted by diabetes. The peak-to-peak amplitude (PPA) was decreased from 3.24 ± 0.7 to 0.9 ± 0.2 mV (p < 0.05), the conduction velocity (CV) of the first component was reduced from 46.78 ± 4.5 to 26.69 ± 3.8 ms (p < 0.05) and chronaxy was increased from 60.43 ± 1.9 to 69.67 ± 1.4 ms (p < 0.05). These parameters were improved in nerves from DCI-treated mice (p < 0.05). PPA in the DCI group was 5.79 ± 0.8 mV (vs. 0.9 ± 0.2 mV-Diab; p < 0.05) and CV was 45.91 ± 3.6 ms (vs. 26.69 ± 3.8 ms-Diab; p < 0.05). Maximal relaxation of the corpus cavernosum evoked by electrical stimulation (2-64 Hz) in the Diab group was 36.4 ± 3.8% compared to 65.4 ± 2.8% in Eugly and 59.3 ± 5.5% in the DCI group (p < 0.05). Maximal contraction obtained in the vas deferens was 38.0 ± 9.2% in Eugly and 11.5 ± 2.6% in Diab (decrease of 69.7%; p < 0.05), compared to 25.2 ± 2.3% in the DCI group (p < 0.05 vs. diabetic). Electron microscopy of the sciatic nerves showed prevention of neuronal damage. Conclusions: DCI has a neuroprotective action in both autonomic and somatic nerves in STZ-induced DN. © 2011 Blackwell Publishing Ltd.
dc.description.firstpage243
dc.description.issuenumber3
dc.description.lastpage250
dc.description.volume13
dc.identifier.doi10.1111/j.1463-1326.2010.01344.x
dc.identifier.issn1463-1326
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37077
dc.relation.ispartofDiabetes, Obesity and Metabolism
dc.rightsAcesso Restrito
dc.subject.otherlanguageAutonomic neuropathy
dc.subject.otherlanguageDiabetic neuropathy
dc.subject.otherlanguageExperimental pharmacology
dc.subject.otherlanguageInositols
dc.subject.otherlanguageMicrovascular disease
dc.subject.otherlanguageSomatic neuropathy
dc.subject.otherlanguageTherapy of diabetic complications
dc.titleChronic treatment with d-chiro-inositol prevents autonomic and somatic neuropathy in STZ-induced diabetic mice
dc.typeArtigo
local.scopus.citations19
local.scopus.eid2-s2.0-78851471705
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78851471705&origin=inward
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