Gene deletion leads to improved valinomycin production by Streptomyces sp. CBMAI 2042

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dc.contributor.authorPaulo B.S.
dc.contributor.authorSigrist R.
dc.contributor.authorAngolini C.F.F.
dc.contributor.authorEberlin M.N.
dc.contributor.authorDe Oliveira L.G.
dc.date.accessioned2024-03-12T23:55:08Z
dc.date.available2024-03-12T23:55:08Z
dc.date.issued2019
dc.description.abstract© 2019 Sociedade Brasileira de QuímicaThe genus Streptomyces represents one of the largest producers of molecules with antibiotic activity. The whole genome sequencing of the endophytic microorganism Streptomyces sp. CBMAI 2042 revealed 35 gene clusters encoding for secondary metabolism including 3 non-ribosomal peptide synthetases (NRPS) and 7 NRPS-hybrids. Combining genome mining and cultivation profile analysis, the depsipeptide ionophore valinomycin was identified as one of the main metabolites produced by this strain. To better understand the metabolic machinery codified in CBMAI 2042 genome an adenylation domain from a hybrid NRPS cluster was deleted through a double crossing knockout experiment. Though the deletion was not plentiful to elucidate the encoded NRP metabolite related to the adenilation domain, an astounding increase of 10.5-fold in valinomycin production was observed in the mutant. These results suggest a metabolic flux redistribution of common substrates as an outcome of a gene target deletion.
dc.description.firstpage673
dc.description.issuenumber3
dc.description.lastpage679
dc.description.volume30
dc.identifier.doi10.21577/0103-5053.20180207
dc.identifier.issn1678-4790
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/35374
dc.relation.ispartofJournal of the Brazilian Chemical Society
dc.rightsAcesso Aberto
dc.subject.otherlanguageGene target deletion
dc.subject.otherlanguageGenome mining
dc.subject.otherlanguageValinomycin
dc.titleGene deletion leads to improved valinomycin production by Streptomyces sp. CBMAI 2042
dc.typeArtigo
local.scopus.citations7
local.scopus.eid2-s2.0-85069792581
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069792581&origin=inward
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