A catalytically inactive Lys49 PLA2 isoform from Bothrops jararacussu venom that stimulates insulin secretion in pancreatic beta cells

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dc.contributor.authorFagundes F.H.R.
dc.contributor.authorAparicio R.
dc.contributor.authorDos Santos M.L.
dc.contributor.authorFilho E.B.S.D.
dc.contributor.authorOliveira S.C.B.
dc.contributor.authorToyama D.O.
dc.contributor.authorToyama M.H.
dc.date.accessioned2024-03-13T01:10:19Z
dc.date.available2024-03-13T01:10:19Z
dc.date.issued2011
dc.description.abstractA new secretory phospholipase A2 (sPLA2) isoform from Bothrops jararacussu venom (BjVIII) has been characterized by causing platelet aggregation, an absent activity in BthTx-I, Prtx-I and PrTx-II sPLA2s. According to our results, BjVIII also enhances insulin release by the pancreatic beta cells. The complete amino acid sequence of the new isoform was determined by Edman degradation and de novo peptide sequencing. These analyses showed a G35K amino acid modification for BjVIII in comparison with BthTx-I, PrTx-I and Prtx-II, a structural difference that has been related to the conflicting biological activities among BjVIII and other Lys49 sPLA2s. The whole set of evidences collected in this work indicates that, besides the C-terminal region and B-wing of PLA2, the calcium binding loop in BjVIII should be considered as an important region, involved in the pharmacological effects of Lys49-sPLA2 isoforms from the Bothrops genus. © 2011 Bentham Science Publishers.
dc.description.firstpage1133
dc.description.issuenumber11
dc.description.lastpage1139
dc.description.volume18
dc.identifier.doi10.2174/092986611797200940
dc.identifier.issn0929-8665
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36934
dc.relation.ispartofProtein and Peptide Letters
dc.rightsAcesso Restrito
dc.subject.otherlanguageBothrops jararacussu
dc.subject.otherlanguageEdman degradation
dc.subject.otherlanguageInsulin secretion
dc.subject.otherlanguageMass spectra
dc.subject.otherlanguagePancreatic beta cells
dc.subject.otherlanguagePLA2
dc.subject.otherlanguagePlatelet aggregation
dc.titleA catalytically inactive Lys49 PLA2 isoform from Bothrops jararacussu venom that stimulates insulin secretion in pancreatic beta cells
dc.typeArtigo
local.scopus.citations10
local.scopus.eid2-s2.0-80053016977
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80053016977&origin=inward
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