Tandem Mass Tag Proteomic Analysis of in Vitro and in Vivo Models of Cutaneous Leishmaniasis Reveals Parasite-Specific and Nonspecific Modulation of Proteins in the Host

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dc.contributor.authorNegrao F.
dc.contributor.authorDiedrich J.K.
dc.contributor.authorGiorgio S.
dc.contributor.authorEberlin M.N.
dc.contributor.authorYates J.R.
dc.date.accessioned2024-03-12T23:50:47Z
dc.date.available2024-03-12T23:50:47Z
dc.date.issued2019
dc.description.abstract© 2019 American Chemical Society.Cutaneous leishmaniasis, the most common form of leishmaniasis, is endemic in several regions of the world, and if not treated properly, it can cause disfiguring scars on the skin. Leishmania spp. infection causes an inflammatory response in its host, and it modulates the host metabolism differently depending on the Leishmania species. Since Leishmania spp. has begun to develop resistance against current therapies, we believe efforts to identify new possibilities for treatment are critical for future control of the disease. Proteomics approaches such as isobaric labeling yield accurate relative quantification of protein abundances and, when combined with chemometrics/statistical analysis, provide robust information about protein modulation across biological conditions. Using a mass spectrometry-based proteomics approach and tandem mass tag labeling, we have investigated protein modulation in murine macrophages (in vitro model) and skin biopsies after exposure to Leishmania spp. (in vivo murine model). Infections induced by L. amazonensis (endemic in the New World) and L. major (endemic in the Old World) were compared to an inflammation model to search for Leishmania-specific and nonspecific protein modulation in the host. After protein extracts obtained from in vitro and in vivo experiments were digested, the resulting peptides were labeled with isobaric tags and analyzed by liquid chromatography-MS (LC-MS). Several proteins that were found to be changed upon infection with Leishmania spp. provide interesting candidates for further investigation into disease mechanism and development of possible immunotherapies.
dc.description.firstpage2136
dc.description.issuenumber12
dc.description.lastpage2147
dc.description.volume5
dc.identifier.doi10.1021/acsinfecdis.9b00275
dc.identifier.issn2373-8227
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/35139
dc.relation.ispartofACS Infectious Diseases
dc.rightsAcesso Restrito
dc.subject.otherlanguagecutaneous leishmaniasis
dc.subject.otherlanguageisobaric labeling proteomics
dc.subject.otherlanguagemass spectrometry
dc.subject.otherlanguageTMT
dc.titleTandem Mass Tag Proteomic Analysis of in Vitro and in Vivo Models of Cutaneous Leishmaniasis Reveals Parasite-Specific and Nonspecific Modulation of Proteins in the Host
dc.typeArtigo
local.scopus.citations7
local.scopus.eid2-s2.0-85074427269
local.scopus.subjectAnimals
local.scopus.subjectDisease Models, Animal
local.scopus.subjectFemale
local.scopus.subjectHost-Parasite Interactions
local.scopus.subjectLeishmania
local.scopus.subjectLeishmaniasis, Cutaneous
local.scopus.subjectMacrophages
local.scopus.subjectMice
local.scopus.subjectMice, Inbred BALB C
local.scopus.subjectProteome
local.scopus.subjectProteomics
local.scopus.subjectTandem Mass Spectrometry
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85074427269&origin=inward
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