Cytokine detection for the diagnosis of chromium allergy Detecção de citocinas no diagnóstico de pacientes alérgicos ao cromo

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dc.contributor.authorMartins L.E.A.M.
dc.contributor.authordos Reis V.M.S.
dc.date.accessioned2024-03-13T01:03:54Z
dc.date.available2024-03-13T01:03:54Z
dc.date.issued2013
dc.description.abstractBACKGROUND: Patch testing remains the gold standard method for the identification of the etiologic agent of allergic contact dermatitis. However, it is a subjective, time-consuming exam whose technique demands special care and which presents some contraindications, which hamper its use. In a recent study, we showed that the proliferation assay can suitably replace patch testing for the diagnosis of chromium allergy, which had been previously demonstrated only for nickel allergy. In this study, we try to refine the method by reducing the incubation period of cultures for lymphocyte proliferation assays in response to chromium. OBJECTIVE: Develop an alternative or complementary diagnostic test for chromium allergic contact dermatitis. METHODS: We compared the production of 9 cytokines (IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17 and RANTES) between 18 chromium-allergic patients and 19 controls. RESULTS: Chromium increased the production of IFN-y, IL-5, IL-2 and IL-13 in allergic patients, but only IL-2 and especially IL-13 helped discriminate allergic patients from controls. The sensitivity, specificity and accuracy found with IL-13 were about 80%. CONCLUSIONS: IL-13 and IL-2 detection may be used to diagnose chromium allergy in 2-day cultures. However, in general, the 6-day cultures seem to be superior for this purpose. © 2013 by Anais Brasileiros de Dermatologia.
dc.description.firstpage719
dc.description.issuenumber5
dc.description.lastpage725
dc.description.volume88
dc.identifier.doi10.1590/abd1806-4841.20132164
dc.identifier.issn0365-0596
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36572
dc.relation.ispartofAnais Brasileiros de Dermatologia
dc.rightsAcesso Aberto
dc.subject.otherlanguageAllergic contact
dc.subject.otherlanguageChromium
dc.subject.otherlanguageContact
dc.subject.otherlanguageCytokines
dc.subject.otherlanguageDermatitis
dc.subject.otherlanguageLaboratory test
dc.subject.otherlanguageLymphocyte activation
dc.titleCytokine detection for the diagnosis of chromium allergy Detecção de citocinas no diagnóstico de pacientes alérgicos ao cromo
dc.typeArtigo
local.scopus.citations4
local.scopus.eid2-s2.0-84891887297
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891887297&origin=inward
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