Fabrication and characterization of physically crosslinked alginate/chitosan-based hydrogel loaded with neomycin for the treatment of skin infections caused by Staphylococcus aureus

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dc.contributor.authorSilva L.D.S.
dc.contributor.authorVila Nova B.G.
dc.contributor.authorSousa C.E.M.D.
dc.contributor.authorSilva R.G.
dc.contributor.authorCarvalho L.R.D.S.
dc.contributor.authorSilva I.S.S.
dc.contributor.authorMoreira P.H.D.A.
dc.contributor.authorCardenas A.F.M.
dc.contributor.authorMonteiro C.D.A.
dc.contributor.authorTofanello A.
dc.contributor.authorGarcia W.
dc.contributor.authorTeixeira C.S.
dc.contributor.authorNascimento da Silva L.C.
dc.date.accessioned2024-07-01T06:10:40Z
dc.date.available2024-07-01T06:10:40Z
dc.date.issued2024
dc.description.abstract© 2024 Elsevier B.V.Staphylococcus aureus is a pathogen widely involved in wound infection due to its ability to release several virulence factors that impair the skin healing process, as well as its mechanism of drug resistance. Herein, sodium alginate and chitosan were combined to produce a hydrogel for topical delivery of neomycin to combat S. aureus associated with skin complications. The hydrogel was formulated by combining sodium alginate (50 mg/mL) and chitosan (50 mg/mL) solutions in a ratio of 9:1 (HBase). Neomycin was added to HBase to achieve a concentration of 0.4 mg/mL (HNeo). The incorporation of neomycin into the product was confirmed by scanning electron microscopy, FTIR and TGA analysis. The hydrogels produced are homogeneous, have a high swelling capacity, and show biocompatibility using erythrocytes and fibroblasts as models. The formulations showed physicochemical and pharmacological stability for 60 days at 4 ± 2 °C. HNeo totally inhibited the growth of S. aureus after 4 h. The antimicrobial effects were confirmed using ex vivo (porcine skin) and in vivo (murine) wound infection models. Furthermore, the HNeo-treated mice showed lower severity scores than those treated with HBase. Taken together, the obtained results present a new low-cost bioproduct with promising applications in treating infected wounds.
dc.description.volume271
dc.identifier.doi10.1016/j.ijbiomac.2024.132577
dc.identifier.issnNone
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/38802
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.rightsAcesso Restrito
dc.subject.otherlanguageBiocompatible hydrogel
dc.subject.otherlanguageInfected wounds
dc.subject.otherlanguagePolymeric formulation
dc.titleFabrication and characterization of physically crosslinked alginate/chitosan-based hydrogel loaded with neomycin for the treatment of skin infections caused by Staphylococcus aureus
dc.typeArtigo
local.scopus.citations2
local.scopus.eid2-s2.0-85194305121
local.scopus.subjectBiocompatible hydrogel
local.scopus.subjectCrosslinked
local.scopus.subjectFabrication and characterizations
local.scopus.subjectInfected wounds
local.scopus.subjectPolymeric formulations
local.scopus.subjectS. aureus
local.scopus.subjectSkin infection
local.scopus.subjectStaphylococcus aureus
local.scopus.subjectVirulence factors
local.scopus.subjectWound infections
local.scopus.subjectAlginates
local.scopus.subjectAnimals
local.scopus.subjectAnti-Bacterial Agents
local.scopus.subjectChitosan
local.scopus.subjectDrug Carriers
local.scopus.subjectHydrogels
local.scopus.subjectMice
local.scopus.subjectNeomycin
local.scopus.subjectSkin
local.scopus.subjectStaphylococcal Infections
local.scopus.subjectStaphylococcal Skin Infections
local.scopus.subjectStaphylococcus aureus
local.scopus.updated2025-04-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85194305121&origin=inward
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