Light induced cytotoxicity of nitrofurantoin toward murine melanoma

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dc.contributor.authorFerreira L.P.
dc.contributor.authorParra G.G.
dc.contributor.authorCodognato D.C.K.
dc.contributor.authorAmado A.M.
dc.contributor.authorDa Silva R.S.
dc.date.accessioned2024-03-13T00:51:06Z
dc.date.available2024-03-13T00:51:06Z
dc.date.issued2017
dc.description.abstract© 2017 The Royal Society of Chemistry and Owner Societies.The cytotoxicity of nitrofurantoin (NFT) in the dark and after light exposure (UVA irradiation, λ = 385 nm) was evaluated in murine melanoma B16F10 cells. NFT induces both cell proliferation and inhibition of cell viability. The dominance of one or the other effect depends on the drug concentration, incubation time (tinc) and irradiation dose. The uptake of NFT in these cells, as well as its photocytotoxicity, reaches saturation after 24 hours of incubation. The mechanism of cell death in the dark is associated with the enzymatic release of nitric oxide (NO). The increase of NFT cytotoxicity under light irradiation is associated with the increase of NO concentration due to photorelease. NO photorelease by NFT in solution was confirmed by chemiluminescence, while NO formation in cells was confirmed by fluorescence microscopy using DAF-2DA, a specific indicator of NO in living cells. The NFT does not enter nuclei, distributing preferentially in the cell cytoplasm, as shown by fluorescence microscopy.
dc.description.firstpage1071
dc.description.issuenumber7
dc.description.lastpage1078
dc.description.volume16
dc.identifier.doi10.1039/c6pp00306k
dc.identifier.issn1474-9092
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/35855
dc.relation.ispartofPhotochemical and Photobiological Sciences
dc.rightsAcesso Restrito
dc.titleLight induced cytotoxicity of nitrofurantoin toward murine melanoma
dc.typeArtigo
local.scopus.citations2
local.scopus.eid2-s2.0-85024092878
local.scopus.subjectAnimals
local.scopus.subjectCell Proliferation
local.scopus.subjectCell Survival
local.scopus.subjectDose-Response Relationship, Drug
local.scopus.subjectMelanoma
local.scopus.subjectMice
local.scopus.subjectNitrofurantoin
local.scopus.subjectPhotosensitizing Agents
local.scopus.subjectStructure-Activity Relationship
local.scopus.subjectTumor Cells, Cultured
local.scopus.subjectUltraviolet Rays
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85024092878&origin=inward
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