Effect of high-fat diet upon inflammatory markers and aortic stiffening in mice

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dc.contributor.authorSantana A.B.C.
dc.contributor.authorSouza Oliveira T.C.D.
dc.contributor.authorBianconi B.L.
dc.contributor.authorBarauna V.G.
dc.contributor.authorSantos E.W.C.O.
dc.contributor.authorAlves T.P.
dc.contributor.authorSilva J.C.S.
dc.contributor.authorFiorino P.
dc.contributor.authorBorelli P.
dc.contributor.authorIrigoyen M.C.C.
dc.contributor.authorKrieger J.E.
dc.contributor.authorLacchini S.
dc.date.accessioned2024-03-13T01:02:16Z
dc.date.available2024-03-13T01:02:16Z
dc.date.issued2014
dc.description.abstractChanges in lifestyle such as increase in high-fat food consumption are an important cause for vascular diseases. The present study aimed to investigate the involvement of ACE and TGF-β in the aorta stiffness induced by high-fat diet. C57BL/6 male mice were divided in two groups according to their diet for 8 weeks: standard diet (ST) and high-fat diet (HF). At the end of the protocol, body weight gain, adipose tissue content, serum lipids and glucose levels, and aorta morphometric and biochemical measurements were performed. Analysis of collagen fibers by picrosirius staining of aorta slices showed that HF diet promoted increase of thin (55%) and thick (100%) collagen fibers deposition and concomitant disorganization of these fibers orientations in the aorta vascular wall (50%). To unravel the mechanism involved, myeloperoxidase (MPO) and angiotensin I converting enzyme (ACE) were evaluated by protein expression and enzyme activity. HF diet increased MPO (90%) and ACE (28%) activities, as well as protein expression of ACE. TGF-β was also increased in aorta tissue of HF diet mice after 8 weeks. Altogether, we have observed that the HF diet-induced aortic stiffening may be associated with increased oxidative stress damage and activation of the RAS in vascular tissue. © 2014 Andre Bento Chaves Santana et al.
dc.description.volume2014
dc.identifier.doi10.1155/2014/914102
dc.identifier.issn2314-6141
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36481
dc.relation.ispartofBioMed Research International
dc.rightsAcesso Aberto
dc.titleEffect of high-fat diet upon inflammatory markers and aortic stiffening in mice
dc.typeArtigo
local.scopus.citations17
local.scopus.eid2-s2.0-84903643117
local.scopus.subjectAnimals
local.scopus.subjectAorta
local.scopus.subjectBiological Markers
local.scopus.subjectDiet, High-Fat
local.scopus.subjectGene Expression Regulation
local.scopus.subjectHumans
local.scopus.subjectInflammation
local.scopus.subjectMice
local.scopus.subjectOxidative Stress
local.scopus.subjectPeptidyl-Dipeptidase A
local.scopus.subjectTransforming Growth Factor beta
local.scopus.subjectVascular Stiffness
local.scopus.subjectWeight Gain
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84903643117&origin=inward
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