Fish oil provides protection against the oxidative stress in pilocarpine model of epilepsy

Página do item simplificado
dc.contributor.authorNejm M.B.
dc.contributor.authorHaidar A.A.
dc.contributor.authorMarques M.J.G.
dc.contributor.authorHirata A.E.
dc.contributor.authorNogueira F.N.
dc.contributor.authorCavalheiro E.A.
dc.contributor.authorScorza F.A.
dc.contributor.authorCysneiros R.M.
dc.date.accessioned2024-03-13T00:56:41Z
dc.date.available2024-03-13T00:56:41Z
dc.date.issued2015
dc.description.abstract© Springer Science+Business Media New York 2015.Temporal lobe epilepsy (TLE), the most common form of epilepsy is often resistant to pharmacological treatment. Neuronal loss observed in epileptic brain may be result of an overproduction of free radicals (oxidative stress). Oxidative stress is characterized by an imbalance between antioxidant defenses and oxidizing agents (free radicals), which can lead to tissue injury. The n-3 PUFAs are important for the development and maintenance of central nervous system functions. Research by our group has shown that chronic treatment with fish oil, immediately after status epilepticus (SE), exhibits both neuroprotective effects and effects on neuroplasticity. The main purpose of this research was to evaluate if fish oil exhibits a protective effect against oxidative stress. Animals were subjected to TLE model by pilocarpine administration. After 3 h of SE they were randomly divided into the following groups: control animals treated daily with vehicle or with 85 mg/kg of fish oil and animals with epilepsy treated daily with vehicle or with 85 mg/kg of fish oil. After 90 days, superoxide anion production, enzymatic activity of superoxide dismutase (SOD) and catalase (CAT) and protein expression of NAD(P)H oxidase subunits (p47PHOX and gp91PHOX) were analyzed. Our results showed evidences that reactive oxygen species are increased in animals with epilepsy and that fish oil supplementation could counteract it. Fish oil supplementation promoted protection against oxidative stress by multiple ways, which involved the reduction of activity and expression of NAD(P)H oxidase subunits and increased the activity and expression of antioxidants enzymes, contributing to well-known neuroprotective effect in epilepsy.
dc.description.firstpage903
dc.description.issuenumber4
dc.description.lastpage909
dc.description.volume30
dc.identifier.doi10.1007/s11011-015-9666-0
dc.identifier.issn1573-7365
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36169
dc.relation.ispartofMetabolic Brain Disease
dc.rightsAcesso Restrito
dc.subject.otherlanguageEpilepsy
dc.subject.otherlanguageFish oil
dc.subject.otherlanguageOxidative stress
dc.subject.otherlanguagePilocarpine
dc.titleFish oil provides protection against the oxidative stress in pilocarpine model of epilepsy
dc.typeArtigo
local.scopus.citations9
local.scopus.eid2-s2.0-84943361134
local.scopus.subjectAnimals
local.scopus.subjectDisease Models, Animal
local.scopus.subjectEpilepsy
local.scopus.subjectFish Oils
local.scopus.subjectMale
local.scopus.subjectNeuroprotective Agents
local.scopus.subjectOxidative Stress
local.scopus.subjectPilocarpine
local.scopus.subjectRats
local.scopus.subjectRats, Wistar
local.scopus.subjectReactive Oxygen Species
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84943361134&origin=inward
Arquivos
Coleções
Artigos de periódico