Renal and cardiovascular effects of Bothrops marajoensis venom and phospholipase A2
Tipo
Artigo
Data de publicação
2010
Periódico
Toxicon
Citações (Scopus)
34
Autores
Evangelista I.L.
Costa Martins A.M.
Falcao Nascimento N.R.
Havt A.
Azul Monteiro Evangelista J.S.
Sa de Noroes T.B.
Toyama M.H.
Diz-Filho E.B.
de Oliveira Toyama D.
Fonteles M.C.
Azul Monteiro H.S.
Costa Martins A.M.
Falcao Nascimento N.R.
Havt A.
Azul Monteiro Evangelista J.S.
Sa de Noroes T.B.
Toyama M.H.
Diz-Filho E.B.
de Oliveira Toyama D.
Fonteles M.C.
Azul Monteiro H.S.
Orientador
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
Bothrops marajoensis is found in the savannah of Marajó Island in the State of Pará and regions of Amapá State, Brazil. The aim of the work was to study the renal and cardiovascular effects of the B. marajoensis venom and phospholipase A2 (PLA2). The venom was fractionated by Protein Pack 5PW. N-terminal amino acid sequencing of sPLA2 showed amino acid identity with other lysine K49 sPLA2s of snake venom. B. marajoensis venom (30 μg/mL) decreased the perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate and sodium tubular transport. PLA2 did not change the renal parameters. The perfusion pressure of the mesenteric bed did not change after infusion of venom. In isolated heart, the venom decreased the force of contraction and increased PP but did not change coronary flow. In the arterial pressure, the venom and PLA2 decreased mean arterial pressure and cardiac frequency. The presence of atrial flutter and late hyperpolarisation reversed, indicating QRS complex arrhythmia and dysfunction in atrial conduction. In conclusion, B. marajoensis venom and PLA2 induce hypotension and bradycardia while simultaneously blocking electrical conduction in the heart. Moreover, the decrease in glomerular filtration rate, urinary flow and electrolyte transport demonstrates physiological changes to the renal system. © 2009 Elsevier Ltd. All rights reserved.