Characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate that does not liquefy the integument of infected larvae

Vieira C.M.; Tuelher E.S.; Valicente F.H.; Wolff J.L.C.

Characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate that does not liquefy the integument of infected larvae

Tipo

Artigo

Data de publicação

2012

Periódico

Journal of Invertebrate Pathology

Citações (Scopus)

21

Autores

Vieira C.M.
Tuelher E.S.
Valicente F.H.
Wolff J.L.C.

Resumo

Here we report the characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate, named SfMNPV-6nd, that does not cause the liquefaction of the host integument. The sequencing of the chitinase A (v-chiA) gene from SfMNPV-6nd revealed that it had a frameshift mutation that greatly reduced size of the putative enzyme. In order to evaluate the suitability of SfMPNV-6nd as a biopesticide, this isolate was compared with the highly virulent SfMNPV-19. Our results showed that the LC50 of the two isolates were not significantly different, but that SfMNPV-6nd took a longer period of time to kill second instar S. frugiperda than SfMNPV-19. © 2012 Elsevier Inc.