BTCI enhances guanylin-induced natriuresis and promotes renal glomerular and tubular effects

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dc.contributor.authorCarvalho A.F.
dc.contributor.authorSantos-Neto
dc.contributor.authorMonteiro H.S.A.
dc.contributor.authorFreitas S.M.
dc.contributor.authorMorhy L.
dc.contributor.authorNascimento N.R.F.
dc.contributor.authorFonteles M.C.
dc.date.accessioned2024-03-13T01:37:44Z
dc.date.available2024-03-13T01:37:44Z
dc.date.issued2008
dc.description.abstractGuanylin and uroguanylin are small cysteine-rich peptides involved in the regulation of fluid and electrolyte homeostasis through binding and activation of guanylyl cyclases signaling molecules expressed in intestine and kidney. Guanylin is less potent than uroguanylin as a natriuretic agent and is degraded in vitro by chymotrypsin due to unique structural features in the bioactive moiety of the peptide. Thus, the aim of this study was to verify whether or not guanylin is degraded by chymotrypsin-like proteases present in the kidney brush-border membranes. The isolated perfused rat kidney assay was used in this regard. Guanylin (0.2 μM) induced no changes in kidney function. However, when pretreated by the black-eyed pea trypsin and chymotrypsin inhibitor (BTCI - 1.0 μM; guanylin - 0.2 μM) it promoted increases in urine flow (ΔUF of 0.25 ± 0.09 mL.g-1/min, P < 0.05) and Na+ excretion (% Δ ENa+ of 18.20 ± 2.17, P < 0.05). BTCI (1.0 μM) also increased %ENa+ (from 22.8 ± 1.30 to 34.4 ± 3.48, P < 0.05, 90 minutes). Furthermore, BTCI (3.0 μM) induced increases in glomerular filtration rate (GFR; from 0.96 ± 0.02 to 1.28 ± 0.02 mL.g-1/min, P < 0.05, 60 minutes). The present paper strongly suggests that chymotrypsin-like proteases play a role in renal metabolism of guanylin and describes for the first time renal effects induced by a member of the Bowman-Birk family of protease inhibitors.
dc.description.firstpage149
dc.description.issuenumber1
dc.description.lastpage154
dc.description.volume68
dc.identifier.doi10.1590/S1519-69842008000100021
dc.identifier.issn1519-6984
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37530
dc.relation.ispartofBrazilian Journal of Biology
dc.rightsAcesso Aberto
dc.subject.otherlanguageBowman-Birk protease inhibitors
dc.subject.otherlanguageBTCI
dc.subject.otherlanguageCyclic GMP
dc.subject.otherlanguageKidney
dc.subject.otherlanguageNatriuresis
dc.subject.otherlanguageVignia unguiculata
dc.titleBTCI enhances guanylin-induced natriuresis and promotes renal glomerular and tubular effects
dc.typeArtigo
local.scopus.citations9
local.scopus.eid2-s2.0-43649086288
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=43649086288&origin=inward
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