Avaliação da atividade das moléculas derivadas de aroilacetonitrila contra a bactéria Pseudomonas aeruginosa
Carregando...
Tipo
TCC
Data de publicação
2022-06
Periódico
Citações (Scopus)
Autores
Araujo, Elis da Silva
Orientador
Delorenzi, Jan Carlo Morais Oliveira Bertassoni
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
A bactéria Pseudomonas aeruginosa considerada importante causadora de ampla resistência bacteriana devido a aspectos intrínsecos e extrínsecos, natural e adquirido, quando exposta a uma variedade de fármacos atualmente utilizada mediante o tratamento contra a ação da bactéria. Um problema de saúde pública que atinge a esfera global, pois há dados que demonstram altas taxas de morbidade e mortalidade associadas à infecção por P. aeruginosa. O que impulsiona novas pesquisas no desenvolvimento de novos fármacos examinando a potencialidade das novas drogas contra o microrganismo. O objetivo do presente trabalho foi verificar a potencialidades de novas moléculas derivados de aroilacetonitrila AQ 1+2, NEG 4, NEG 1+2, NEG 3, NEG 5, NEG 511 e NEG 512 com ação específica em P. aeruginosa provenientes de banco de cepas diferentes do ano 2017 e 2018. Foram realizados testes de sensibilidade microbiana com as moléculas nas concentrações de 10μg/ml, 5μg/ml, 2,5μg/ml, 1,25μg/ml e 0,75μg/ml e os resultados demonstraram que as moléculas AQ 1+2, NEG 3, NEG 4, NEG 1+2 e NEG 5 em todas as concentrações testadas não consegue impedir o crescimento bacteriano. Contudo a molécula NEG 511 nas concentrações de 10μg/ml e 5μg/ml obteve resultados satisfatórios inibindo o crescimento bacteriano e a molécula NEG 512 na concentração de 10μg/ml conseguiu impedir o crescimento da bactéria e na concentração de 5μg/ml os resultados analisados mostraram que a molécula consegue inibir o crescimento da bactéria oriunda do banco de cepa 2017, porém o resultado obtido com a bactéria proveniente do banco de cepa 2018 ocorre o seu desenvolvimento. Esse resultado demonstra uma mudança na expressão gênica do DNA bacteriano por ter sido extraída de locais diferentes. O presente trabalho demonstrou a relevância da pesquisa na área de resistência bacteriana, na criação de novas condutas terapêuticas e aprimoramento da tecnologia com a finalidade de minimizar esse problema de Saúde Pública.
The bacterium Pseudomonas aeruginosa is considered an important cause of widespread bacterial resistance due to intrinsic and extrinsic aspects, both natural and acquired, when exposed to a variety of drugs currently used in the treatment against the action of bacterium. A public health problem that affects the global sphere, as there are data that demonstrate high rates of morbidity and mortality associated with infection by P. aeruginosa. What drives new research in the development of new drugs examining the potential of new drugs against the microorganism. The objective of the present work was to verify the potential of new molecules derived from aroylacetonitrile AQ 1+2, NEG 4, NEG 1+2, NEG 3, NEG 5, NEG 511, and NEG 512 with specific action on P. aeruginosa from bank of different strains from the year 2017 and 2018. Microbial sensitivity tests were performed with the molecules at concentrations of 10μg/ml, 5μg/ml, 2,5μg/ml, 1,25μg/ml and 0,75μg/ml and the results demonstrated thet the molecules AQ 1+2, NEG 3, NEG 4, NEG 1+2, and NEG 5 at all tested concentrations cannot prevent bacterial growth. However, the molecule NEG 511 in the concentrations of 10μg/ml and 5μg/ml obtained satisfactory results inhibiting the bacterial growth and the molecule NEG 512 in the concentration of 5μg/ml the analyzed results showed that the molecule can inhibit the growth of the bactéria from the 2017 strain bank, but the result obtained with the 2018 strain bank bactéria occurs its development. This result demonstrates a change in bacterial DNA gene expression. As it was extracted from diferentes sites. The presente work demonstrated the relevance of research in the area of bacterial resistance, in the creation of new therapeutic approaches and improvement of technology in order to minimize this Public Health problem.
The bacterium Pseudomonas aeruginosa is considered an important cause of widespread bacterial resistance due to intrinsic and extrinsic aspects, both natural and acquired, when exposed to a variety of drugs currently used in the treatment against the action of bacterium. A public health problem that affects the global sphere, as there are data that demonstrate high rates of morbidity and mortality associated with infection by P. aeruginosa. What drives new research in the development of new drugs examining the potential of new drugs against the microorganism. The objective of the present work was to verify the potential of new molecules derived from aroylacetonitrile AQ 1+2, NEG 4, NEG 1+2, NEG 3, NEG 5, NEG 511, and NEG 512 with specific action on P. aeruginosa from bank of different strains from the year 2017 and 2018. Microbial sensitivity tests were performed with the molecules at concentrations of 10μg/ml, 5μg/ml, 2,5μg/ml, 1,25μg/ml and 0,75μg/ml and the results demonstrated thet the molecules AQ 1+2, NEG 3, NEG 4, NEG 1+2, and NEG 5 at all tested concentrations cannot prevent bacterial growth. However, the molecule NEG 511 in the concentrations of 10μg/ml and 5μg/ml obtained satisfactory results inhibiting the bacterial growth and the molecule NEG 512 in the concentration of 5μg/ml the analyzed results showed that the molecule can inhibit the growth of the bactéria from the 2017 strain bank, but the result obtained with the 2018 strain bank bactéria occurs its development. This result demonstrates a change in bacterial DNA gene expression. As it was extracted from diferentes sites. The presente work demonstrated the relevance of research in the area of bacterial resistance, in the creation of new therapeutic approaches and improvement of technology in order to minimize this Public Health problem.
Descrição
Palavras-chave
Pseudomonas aeruginosa , resistência bacteriana , antimicrobiano , bacterial resistance , antimicrobial