In situ Desi-MSI lipidomic profiles of breast cancer molecular subtypes and precursor lesions

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dc.contributor.authorSantoro A.L.
dc.contributor.authorDrummond R.D.
dc.contributor.authorSilva I.T.
dc.contributor.authorFerreira S.S.
dc.contributor.authorJuliano L.
dc.contributor.authorVendramini P.H.
dc.contributor.authorda Costa Lemos M.B.
dc.contributor.authorEberlin M.N.
dc.contributor.authorAndrade V.P.
dc.date.accessioned2024-03-12T23:48:31Z
dc.date.available2024-03-12T23:48:31Z
dc.date.issued2020
dc.description.abstract© 2020 American Association for Cancer Research.Clinically meaningful molecular subtypes for classification of breast cancers have been established, however, initiation and progression of these subtypes remain poorly understood. The recent development of desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) facilitates the convergence of analytical chemistry and traditional pathology, allowing chemical profiling with minimal tissue pretreatment in frozen samples. Here, we characterized the chemical composition of molecular subtypes of breast cancer with DESI-MSI. Regions of interest were identified, including invasive breast cancer (IBC), ductal carcinoma in situ (DCIS), and adjacent benign tissue (ABT), and metabolomic profiles at 200 mm elaborated using Biomap software and the Lasso method. Top ions identified in IBC regions included polyunsaturated fatty acids, deprotonated glycerophospholipids, and sphingolipids. Highly saturated lipids, as well as antioxidant molecules [taurine (m/z 124.0068), uric acid (m/z 167.0210), ascorbic acid (m/z 175.0241), and glutathione (m/z 306.0765)], were able to distinguish IBC from ABT. Moreover, luminal B and triple-negative subtypes showed more complex lipid profiles compared with luminal A and HER2 subtypes. DCIS and IBC were distinguished on the basis of cell signaling and apoptosis-related ions [fatty acids (341.2100 and 382.3736 m/z) and glycerophospholipids (PE (P-16:0/22:6, m/z 746.5099, and PS (38:3), m/z 812.5440)]. In summary, DESI-MSI identified distinct lipid composition between DCIS and IBC and across molecular subtypes of breast cancer, with potential implications for breast cancer pathogenesis.
dc.description.firstpage1246
dc.description.issuenumber6
dc.description.lastpage1257
dc.description.volume80
dc.identifier.doi10.1158/0008-5472.CAN-18-3574
dc.identifier.issn1538-7445
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/35010
dc.relation.ispartofCancer Research
dc.rightsAcesso Aberto
dc.titleIn situ Desi-MSI lipidomic profiles of breast cancer molecular subtypes and precursor lesions
dc.typeArtigo
local.scopus.citations64
local.scopus.eid2-s2.0-85081944823
local.scopus.subjectBiomarkers, Tumor
local.scopus.subjectBreast
local.scopus.subjectBreast Neoplasms
local.scopus.subjectCarcinoma, Ductal, Breast
local.scopus.subjectCarcinoma, Intraductal, Noninfiltrating
local.scopus.subjectDisease Progression
local.scopus.subjectFemale
local.scopus.subjectHumans
local.scopus.subjectLipid Metabolism
local.scopus.subjectLipidomics
local.scopus.subjectLipids
local.scopus.subjectPrecancerous Conditions
local.scopus.subjectSpectrometry, Mass, Electrospray Ionization
local.scopus.subjectTandem Mass Spectrometry
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081944823&origin=inward
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