Purification and renal effects of phospholipase A2 isolated from Bothrops insularis venom

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dc.contributor.authorMachado Braga M.D.
dc.contributor.authorCosta Martins A.M.
dc.contributor.authorAlves C.D.
dc.contributor.authorde Menezes D.B.
dc.contributor.authorMartins R.D.
dc.contributor.authorFerreira Barbosa P.S.
dc.contributor.authorde Sousa Oliveira I.M.
dc.contributor.authorToyama M.H.
dc.contributor.authorToyama D.O.
dc.contributor.authordos Santos Diz Filho E.B.
dc.contributor.authorRamos Fagundes F.H.
dc.contributor.authorFonteles M.C.
dc.contributor.authorAzul Monteiro H.S.
dc.date.accessioned2024-03-13T01:37:47Z
dc.date.available2024-03-13T01:37:47Z
dc.date.issued2008
dc.description.abstractBothrops insularis venom contains a variety of substances presumably responsible for several pharmacological effects. We investigated the biochemical and biological effects of phospholipase A2 protein isolated from B. insularis venom and the chromatographic profile showed 7 main fractions and the main phospholipase A2 (PLA2) enzymatic activity was detected in fractions IV and V. Fraction IV was submitted to a new chromatographic procedure on ion exchange chromatography, which allowed the elution of 5 main fractions designated as IV-1 to IV-5, from which IV-4 constituted the main fraction. The molecular homogeneity of this fraction was characterized by high-performance liquid chromatography (HPLC) and demonstrated by mass spectrometry (MS), which showed a molecular mass of 13984.20 Da; its N-terminal sequence presented a high amino acid identity (up to 95%) with the PLA2 of Bothrops jararaca and Bothrops asper. Phospholipase A2 isolated from B. insularis (Bi PLA2 ) venom (10 μg/mL) was also studied as to its effect on the renal function of isolated perfused kidneys of Wistar rats (n=6). Bi PLA2 increased perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF) and glomerular filtration rate (GFR). Sodium (%TNa+) and chloride tubular reabsorption (%TCl-) decreased at 120 min, without alteration in potassium transport. In conclusion, PLA2 isolated from B. insularis venom promoted renal alterations in the isolated perfused rat kidney. © 2007 Elsevier Ltd. All rights reserved.
dc.description.firstpage181
dc.description.issuenumber2
dc.description.lastpage190
dc.description.volume51
dc.identifier.doi10.1016/j.toxicon.2007.08.017
dc.identifier.issn0041-0101
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37533
dc.relation.ispartofToxicon
dc.rightsAcesso Restrito
dc.subject.otherlanguageBothrops insularis
dc.subject.otherlanguagePhospholipase A2
dc.subject.otherlanguageRenal biological activity
dc.titlePurification and renal effects of phospholipase A2 isolated from Bothrops insularis venom
dc.typeArtigo
local.scopus.citations13
local.scopus.eid2-s2.0-38349011304
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=38349011304&origin=inward
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